PUBLICATION

A genetically encoded reporter of synaptic activity in vivo

Authors
Dreosti, E., Odermatt, B., Dorostkar, M.M., and Lagnado, L.
ID
ZDB-PUB-091120-39
Date
2009
Source
Nature Methods   6(12): 883-889 (Journal)
Registered Authors
Lagnado, Leon, Odermatt, Benjamin
Keywords
none
MeSH Terms
  • Action Potentials
  • Animals
  • Calcium/metabolism*
  • Synapses/metabolism
  • Synapses/physiology*
  • Zebrafish
PubMed
19898484 Full text @ Nat. Methods
Abstract
To image synaptic activity within neural circuits, we tethered the genetically encoded calcium indicator (GECI) GCaMP2 to synaptic vesicles by fusion to synaptophysin. The resulting reporter, SyGCaMP2, detected the electrical activity of neurons with two advantages over existing cytoplasmic GECIs: it identified the locations of synapses and had a linear response over a wider range of spike frequencies. Simulations and experimental measurements indicated that linearity arises because SyGCaMP2 samples the brief calcium transient passing through the presynaptic compartment close to voltage-sensitive calcium channels rather than changes in bulk calcium concentration. In vivo imaging in zebrafish demonstrated that SyGCaMP2 can assess electrical activity in conventional synapses of spiking neurons in the optic tectum and graded voltage signals transmitted by ribbon synapses of retinal bipolar cells. Localizing a GECI to synaptic terminals provides a strategy for monitoring activity across large groups of neurons at the level of individual synapses.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping