PUBLICATION
Molecular cloning and functional analysis of the zebrafish follicle-stimulating hormone (FSH)beta promoter
- Authors
- Chen, J.Y., Chiou, M.J., Chen, L.K., and Wu, J.L.
- ID
- ZDB-PUB-091120-34
- Date
- 2010
- Source
- Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology 155(2): 155-163 (Journal)
- Registered Authors
- Chen, Jyh-Yih, Wu, Jen-Leih
- Keywords
- Follicle-stimulating hormone gene, Promoter analysis, Zebrafish
- MeSH Terms
-
- Signal Transduction
- Zebrafish/embryology
- Zebrafish/genetics*
- Zebrafish/physiology
- Pituitary Gland/metabolism
- Female
- Follicle Stimulating Hormone, beta Subunit/genetics*
- Follicle Stimulating Hormone, beta Subunit/metabolism
- Bioreactors
- Mitogen-Activated Protein Kinase 1/metabolism
- Fertilization
- Time Factors
- Ovum/physiology
- Animals
- Enzyme Activation
- Ovary/cytology
- Ovary/drug effects
- Ovary/metabolism
- Cell Line
- Animals, Genetically Modified
- Mitogen-Activated Protein Kinase 3/metabolism
- Cloning, Molecular
- Protein Kinase C/metabolism
- Gonadotropin-Releasing Hormone/metabolism
- Calcium/pharmacology
- Embryo, Nonmammalian/metabolism
- Gene Expression Regulation/drug effects
- Promoter Regions, Genetic/genetics*
- PubMed
- 19896554 Full text @ Comp. Biochem. Physiol. B Biochem. Mol. Biol.
Citation
Chen, J.Y., Chiou, M.J., Chen, L.K., and Wu, J.L. (2010) Molecular cloning and functional analysis of the zebrafish follicle-stimulating hormone (FSH)beta promoter. Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology. 155(2):155-163.
Abstract
In the present study, we cloned and characterized a zebrafish follicle-stimulating hormone (zfFSH)beta promoter with deletion fragments transfected into a tilapia ovary (TO2) cell line, and demonstrated that the zfFSHbeta promoter responded to 6h of gonadotropin-releasing hormone (GnRH) treatment by activating calcium influx and protein kinase C (PKC), but after 24h, GnRH induction was generated by activation of extracellular-regulated kinase (ERK)1/2 and repression by PKC. Furthermore, to study the promoter-specific expression, we constructed a series of FSHbeta (4.0-, 3.0-, 2.0-, and 1.0-kb) promoter-driven green fluorescent protein (GFP) fragments encoding the GFP complementary DNA transgene which was microinjected into zebrafish embryos. Morphological studies of transgenic zebrafish indicated that the FSHbeta promoter-driven GFP transcripts appeared in the heart, skin, and vertebrae.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping