PUBLICATION
Activating transcription factor 3 (ATF3) expression in the neural retina and optic nerve of zebrafish during optic nerve regeneration
- Authors
- Saul, K.E., Koke, J.R., and García, D.M.
- ID
- ZDB-PUB-091120-33
- Date
- 2010
- Source
- Comparative biochemistry and physiology. Part A, Molecular & integrative physiology 155(2): 172-182 (Journal)
- Registered Authors
- Keywords
- Optic nerve regeneration, Zebrafish, ATF3, Differential gene expression, cAMP, Oligodendrocytes, Astrocytes, Retinal ganglion cells
- Datasets
- GEO:GSE17854
- MeSH Terms
-
- Activating Transcription Factor 3/genetics*
- Activating Transcription Factor 3/metabolism
- Animals
- Gene Expression Profiling
- In Situ Hybridization
- Microscopy, Fluorescence
- Nerve Regeneration/genetics*
- Nerve Regeneration/physiology
- Oligonucleotide Array Sequence Analysis
- Optic Nerve/metabolism*
- Optic Nerve/physiopathology
- Optic Nerve Injuries/genetics
- Optic Nerve Injuries/physiopathology
- Retina/metabolism*
- Reverse Transcriptase Polymerase Chain Reaction
- Zebrafish/genetics*
- Zebrafish/metabolism
- PubMed
- 19896551 Full text @ Comp. Biochem. Physiol. A Mol. Integr. Physiol.
Citation
Saul, K.E., Koke, J.R., and García, D.M. (2010) Activating transcription factor 3 (ATF3) expression in the neural retina and optic nerve of zebrafish during optic nerve regeneration. Comparative biochemistry and physiology. Part A, Molecular & integrative physiology. 155(2):172-182.
Abstract
Fish, unlike mammals, can regenerate axons in the optic nerve following optic nerve injury. We hypothesized that using microarray analysis to compare gene expression in fish which had experienced optic nerve lesion to fish which had undergone a similar operation but without optic nerve injury would reveal genes specifically involved in responding to optic nerve injury (including repair), reducing detection of genes involved in the general stress and inflammatory responses. We discovered 120 genes were significantly (minimally two-fold with a P-value
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping