PUBLICATION
The Zn Finger protein Iguana impacts Hedgehog signaling by promoting ciliogenesis
- Authors
- Glazer, A., Wilkinson, A., Backer, C.B., Lapan, S., Gutzman, J.H., Cheeseman, I.M., and Reddien, P.W.
- ID
- ZDB-PUB-091101-9
- Date
- 2010
- Source
- Developmental Biology 337(1): 148-156 (Journal)
- Registered Authors
- Gutzman, Jennifer
- Keywords
- Planarians, Cilia, Hedgehog, Iguana, Zebrafish
- MeSH Terms
-
- Adaptor Proteins, Signal Transducing/physiology*
- Animals
- Carrier Proteins/physiology*
- Cilia/physiology*
- HeLa Cells
- Hedgehog Proteins/physiology*
- Humans
- Planarians/physiology*
- Signal Transduction/physiology*
- Zebrafish
- Zinc Fingers/physiology*
- PubMed
- 19852954 Full text @ Dev. Biol.
Citation
Glazer, A., Wilkinson, A., Backer, C.B., Lapan, S., Gutzman, J.H., Cheeseman, I.M., and Reddien, P.W. (2010) The Zn Finger protein Iguana impacts Hedgehog signaling by promoting ciliogenesis. Developmental Biology. 337(1):148-156.
Abstract
Hedgehog signaling is critical for metazoan development and requires cilia for pathway activity. The gene iguana was discovered in zebrafish as required for Hedgehog signaling, and encodes a novel Zn finger protein. Planarians are flatworms with robust regenerative capacities and that utilize epidermal cilia for locomotion. RNA interference of Smed-iguana in the planarian S. mediterranea caused cilia loss and failure to regenerate new cilia, but did not cause defects similar to those observed in hedgehog(RNAi) animals. Smed-iguana gene expression was also similar in pattern to the expression of multiple other ciliogenesis genes, but was not required for expression of these ciliogenesis genes. iguana-defective zebrafish had too few motile cilia in pronephric ducts and in Kupffer's vesicle. Kupffer's vesicle promotes left-right asymmetry and iguana mutant embryos had left-right asymmetry defects. Finally, human Iguana proteins (dZIP1 and dZIP1L) localize to the basal bodies of primary cilia and, together, are required for primary cilia formation. Our results indicate that a critical and broadly conserved function for Iguana is in ciliogenesis and that this function has come to be required for Hedgehog signaling in vertebrates.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping