PUBLICATION
Mta3-NuRD complex is a master regulator for initiation of primitive hematopoiesis in vertebrate embryos
- Authors
- Li, X., Jia, S., Wang, S., Wang, Y., and Meng, A.
- ID
- ZDB-PUB-091101-28
- Date
- 2009
- Source
- Blood 114(27): 5464-5472 (Journal)
- Registered Authors
- Jia, Shunji, Li, Xiang, Meng, Anming
- Keywords
- none
- MeSH Terms
-
- Acetylation
- Animals
- Animals, Genetically Modified
- Embryo, Nonmammalian/blood supply*
- Embryo, Nonmammalian/embryology
- Embryo, Nonmammalian/metabolism
- Gene Expression Regulation, Developmental
- Gene Knockdown Techniques
- Hematopoiesis/drug effects
- Hematopoiesis/genetics
- Hematopoiesis/physiology*
- Histone Deacetylase 1/antagonists & inhibitors
- Histone Deacetylase 1/genetics
- Histone Deacetylase 1/metabolism
- Histone Deacetylase Inhibitors/pharmacology
- Humans
- Hydroxamic Acids/pharmacology
- In Situ Hybridization
- Mi-2 Nucleosome Remodeling and Deacetylase Complex/genetics
- Mi-2 Nucleosome Remodeling and Deacetylase Complex/metabolism*
- Neovascularization, Physiologic/genetics
- Neovascularization, Physiologic/physiology
- Repressor Proteins/genetics
- Repressor Proteins/metabolism*
- Time Factors
- Valproic Acid/pharmacology
- Zebrafish/embryology
- Zebrafish/genetics
- Zebrafish/metabolism
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 19864643 Full text @ Blood
Citation
Li, X., Jia, S., Wang, S., Wang, Y., and Meng, A. (2009) Mta3-NuRD complex is a master regulator for initiation of primitive hematopoiesis in vertebrate embryos. Blood. 114(27):5464-5472.
Abstract
Metastasis-associated antigen (Mta) 1/2/3 are components of NuRD complexes and have been found to play roles in embryonic development and homeostasis. However, their functions in primitive hematopoiesis are unknown. In this study, we demonstrate that knockdown of mta3 by antisense morpholinos abolishes primitive hematopoietic lineages and causes abnormal angiogenesis in zebrafish embryos. However, the expression of the pronephric duct and paraxial mesoderm markers is unaltered and the specification of angioblasts is unaffected in mta3 morphants. The results suggest that mta3 is specifically required for primitive hematopoiesis. Furthermore, inhibition of deacetylase activity with the inhibitors VPA or TSA in zebrafish embryos completely blocks primitive hematopoiesis, resulting in hematopoietic defects almost identical to those seen in mta3 morphants. Importantly, overexpression of scl or scl and lmo2, two master genes for primitive hematopoiesis, is able to overturn effects of mta3 knockdown or VPA/TSA treatment; and overexpression of mta3, human MBD3 or HDAC1, two other components of NuRD complex, enhances the expression of scl and lmo2 in the posterior lateral plate mesoderm during early primitive hematopoiesis. We conclude that Mta3-NuRD complex is essential for the initiation of primitive hematopoiesis. Thus, our findings provide new insight into the regulatory hierarchy of primitive hematopoiesis in vertebrates.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping