PUBLICATION
Cell Fate and Differentiation of the Developing Ocular Lens
- Authors
- Greiling, T.M., Aose, M., and Clark, J.I.
- ID
- ZDB-PUB-091023-49
- Date
- 2010
- Source
- Investigative ophthalmology & visual science 51(3): 1540-1546 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Apoptosis/physiology
- Cell Differentiation/physiology*
- Cell Movement/physiology
- Cell Proliferation
- Epithelial Cells/cytology*
- In Situ Nick-End Labeling
- Lens, Crystalline/cytology*
- Lens, Crystalline/embryology*
- Microscopy, Fluorescence, Multiphoton/methods
- Organogenesis/physiology*
- Zebrafish/embryology*
- PubMed
- 19834024 Full text @ Invest. Ophthalmol. Vis. Sci.
Citation
Greiling, T.M., Aose, M., and Clark, J.I. (2010) Cell Fate and Differentiation of the Developing Ocular Lens. Investigative ophthalmology & visual science. 51(3):1540-1546.
Abstract
Purpose: Even though development in zebrafish does not include formation of a lens vesicle, our hypothesis is that the processes of cell differentiation are similar in zebrafish and mammals and determine cell fates in lens. Methods: 2-photon live-embryo imaging was used to follow individual fluorescently labeled cells in real-time from the placode stage at 16 hours post-fertilization (hpf) until obvious morphological differentiation into epithelium or fiber cells had occurred at approximately 28hpf. Immunohistochemistry was used to label proliferating, differentiating, and apoptotic cells. Results: Similar to the mammal, cells in the teleost peripheral lens placode migrated to the anterior lens mass and differentiated into an anterior epithelium. Cells in the central lens placode migrated into the posterior lens mass and differentiated into primary fiber cells. Anterior and posterior polarization in the zebrafish lens mass was similar to mammalian lens vesicle polarization. Primary fiber cell differentiation was apparent at approximately 21hpf, prior to separation of the lens from the surface ectoderm, as evidenced by cell elongation, exit from the cell cycle, and expression of Zl-1, a marker for fiber differentiation. TUNEL labeling demonstrated that apoptosis was not a primary mechanism for lens separation from the surface ectoderm. Conclusion: Despite the absence of a lens vesicle in the zebrafish embryo, lens organogenesis appears to be well conserved among vertebrates. The results using 3D live embryo imaging of zebrafish development showed minimal differences and strong similarities in the fate of cells in the zebrafish and mammalian lens placode.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping