PUBLICATION
Region-Based PDEs for Cells Counting and Segmentation in 3D+Time Images of Vertebrate Early Embryogenesis
- Authors
- Rizzi, B., and Sarti, A.
- ID
- ZDB-PUB-091023-45
- Date
- 2009
- Source
- International journal of biomedical imaging 2009: 968986 (Journal)
- Registered Authors
- Rizzi, Barbara
- Keywords
- none
- MeSH Terms
- none
- PubMed
- 19830251 Full text @ Int. J. Biomed. Imaging
Citation
Rizzi, B., and Sarti, A. (2009) Region-Based PDEs for Cells Counting and Segmentation in 3D+Time Images of Vertebrate Early Embryogenesis. International journal of biomedical imaging. 2009:968986.
Abstract
This paper is devoted to the segmentation of cell nuclei from time lapse confocal microscopy images, taken throughout early Zebrafish embryogenesis. The segmentation allows to identify and quantify the number of cells in the animal model. This kind of information is relevant to estimate important biological parameters such as the cell proliferation rate in time and space. Our approach is based on the active contour model without edges. We compare two different formulations of the model equation and evaluate their performances in segmenting nuclei of different shapes and sizes. Qualitative and quantitative comparisons are performed on both synthetic and real data, by means of suitable gold standard. The best approach is then applied on a number of time lapses for the segmentation and counting of cells during the development of a zebrafish embryo between the sphere and the shield stage.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping