PUBLICATION
In-vivo Characterization of Human Dilated Cardiomyopathy Genes in Zebrafish
- Authors
- Vogel, B., Meder, B., Just, S., Laufer, C., Berger, I., Weber, S., Katus, H.A., and Rottbauer, W.
- ID
- ZDB-PUB-091023-12
- Date
- 2009
- Source
- Biochemical and Biophysical Research Communications 390(3): 516-522 (Journal)
- Registered Authors
- Berger, Ina, Just, Steffen, Laufer, Christina, Meder, Benjamin, Rottbauer, Wolfgang, Vogel, Britta, Weber, Sabrina
- Keywords
- genetics, dilated cardiomyopathy, zebrafish
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Cardiomyopathy, Dilated/genetics*
- Cloning, Molecular
- Conserved Sequence
- Gene Expression
- Gene Knockdown Techniques
- Humans
- Myocytes, Cardiac/metabolism
- Myocytes, Cardiac/ultrastructure*
- Zebrafish/abnormalities
- Zebrafish/genetics*
- Zebrafish Proteins/genetics*
- PubMed
- 19800866 Full text @ Biochem. Biophys. Res. Commun.
Citation
Vogel, B., Meder, B., Just, S., Laufer, C., Berger, I., Weber, S., Katus, H.A., and Rottbauer, W. (2009) In-vivo Characterization of Human Dilated Cardiomyopathy Genes in Zebrafish. Biochemical and Biophysical Research Communications. 390(3):516-522.
Abstract
Due to lack of families suitable for linkage analysis and positional cloning most of the genetic causes of human dilated cardiomyopathy (DCM) are still unknown. To facilitate rapid identification and validation of novel DCM disease genes appropriate animal models are needed. To assess here for the first time whether the zebrafish is a suitable model organism to validate DCM candidate genes using antisense knock-down strategies, we inactivated in zebrafish known human DCM disease genes and then evaluated the resulting cardiac phenotypes. Consistently, knock-down of the here selected human DCM genes leads to severe heart failure with impairment of systolic cardiac function in zebrafish. Furthermore, gene-specific differences which are also seen in human DCM can be reliably reproduced in the zebrafish model. Our results indicate that the zebrafish is a suitable model organism to rapidly evaluate novel DCM disease genes in-vivo.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping