PUBLICATION

Genetic and optical targeting of neural circuits and behavior-zebrafish in the spotlight

Authors
Baier, H., and Scott, E.K.
ID
ZDB-PUB-090929-15
Date
2009
Source
Current opinion in neurobiology   19(5): 553-560 (Review)
Registered Authors
Baier, Herwig, Scott, Ethan
Keywords
none
MeSH Terms
  • Animals
  • Behavior, Animal/physiology*
  • Brain/physiology
  • Genetic Techniques*/instrumentation
  • Neural Pathways/physiology
  • Neurons/physiology*
  • Optics and Photonics/instrumentation
  • Optics and Photonics/methods*
  • Zebrafish/genetics*
  • Zebrafish/physiology*
PubMed
19781935 Full text @ Curr. Opin. Neurobiol.
Abstract
Methods to label neurons and to monitor their activity with genetically encoded fluorescent reporters have been a staple of neuroscience research for several years. The recent introduction of photoswitchable ion channels and pumps, such as channelrhodopsin (ChR2), halorhodopsin (NpHR), and light-gated glutamate receptor (LiGluR), is enabling remote optical manipulation of neuronal activity. The translucent brains of zebrafish offer superior experimental conditions for optogenetic approaches in vivo. Enhancer and gene trapping approaches have generated hundreds of Gal4 driver lines in which the expression of UAS-linked effectors can be targeted to subpopulations of neurons. Local photoactivation of genetically targeted LiGluR, ChR2, or NpHR has uncovered novel functions for specific areas and cell types in zebrafish behavior. Because the manipulation is restricted to times and places where genetics (cell types) and optics (beams of light) intersect, this method affords excellent resolving power for the functional analysis of neural circuitry.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes