PUBLICATION

Impact of NAD(P)H:Quinone Oxidoreductase-1 on Pigmentation

Authors
Choi, T.Y., Sohn, K.C., Kim, J.H., Kim, S.M., Kim, C.H., Hwang, J.S., Lee, J.H., Kim, C.D., and Yoon, T.J.
ID
ZDB-PUB-090921-22
Date
2010
Source
The Journal of investigative dermatology   130(3): 784-792 (Journal)
Registered Authors
Choi, Tae-Young, Kim, Cheol-Hee
Keywords
none
MeSH Terms
  • Adenoviridae/genetics
  • Alkaloids/biosynthesis
  • Animals
  • Biomarkers, Tumor/metabolism
  • Cell Line, Tumor
  • Gene Expression/physiology
  • Humans
  • Lymph Nodes/cytology
  • Melanins/biosynthesis
  • Melanocytes/cytology
  • Melanocytes/physiology
  • Melanoma/metabolism
  • Melanoma/physiopathology
  • Monophenol Monooxygenase/metabolism
  • NAD(P)H Dehydrogenase (Quinone)/genetics*
  • NAD(P)H Dehydrogenase (Quinone)/metabolism*
  • Recombinant Proteins/genetics
  • Recombinant Proteins/metabolism
  • Skin Neoplasms/metabolism
  • Skin Neoplasms/physiopathology
  • Skin Pigmentation/genetics*
  • Zebrafish
PubMed
19759547 Full text @ J. Invest. Dermatol.
Abstract
We obtained metastasized melanoma tissue from a primary acral lentiginous melanoma (ALM) patient and established a melanoma cell line named primary culture of melanoma cell derived from lymph node (PML)-1. PML-1 cells had a light brown color and decreased the expression of melanogenesis markers, including tyrosinase (TYR), microphthalmia-associated transcription factor, and tyrosinase-related protein-1. To identify genes differentially regulated in PML-1 melanoma cells, we performed DNA microarray and two-dimensional matrix-assisted laser desorption ionization-time of flight mass spectrometry analyses. Among the candidate genes identified, we chose NAD(P)H:quinone oxidoreductase-1 (NQO1) for further study. Reverse transcription-PCR and western blot analyses showed that NQO1 was markedly decreased in PML-1 cells and in several amelanotic melanoma cell lines. To investigate whether NQO1 affects the melanogenesis, we treated the cultured normal human melanocytes (NHMC) and zebrafish with NQO1 inhibitors, ES936 and dicoumarol. Interestingly, melanogenesis was significantly decreased by the addition of NQO1 inhibitors in both NHMC and zebrafish models. In contrast, overexpression of NQO1 using a recombinant adenovirus clearly induced melanogenesis, concomitantly with an increase of TYR protein level. These results suggest that NQO1 is a positive regulator of the pigmentation process.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes