PUBLICATION
Glial cell line-derived neurotrophic factor in Purkinje cells of adult zebrafish: An autocrine mode of action?
- Authors
- Facello, B., Castaldo, L., De Martino, L., and Lucini, C.
- ID
- ZDB-PUB-090914-34
- Date
- 2009
- Source
- Neuroscience letters 465(2): 133-137 (Journal)
- Registered Authors
- Keywords
- Cerebellum, GDNF, GFRalpha1, RET, Danio rerio, Fish
- MeSH Terms
-
- Glial Cell Line-Derived Neurotrophic Factor Receptors/metabolism
- RNA, Messenger/metabolism
- Zebrafish Proteins/metabolism
- Immunohistochemistry
- Zebrafish
- Animals
- Proto-Oncogene Proteins c-ret/metabolism
- Purkinje Cells/metabolism*
- Glial Cell Line-Derived Neurotrophic Factor/metabolism*
- Cerebellum/metabolism*
- Fructose-Bisphosphate Aldolase/metabolism
- Reverse Transcriptase Polymerase Chain Reaction
- PubMed
- 19732805 Full text @ Neurosci. Lett.
Citation
Facello, B., Castaldo, L., De Martino, L., and Lucini, C. (2009) Glial cell line-derived neurotrophic factor in Purkinje cells of adult zebrafish: An autocrine mode of action?. Neuroscience letters. 465(2):133-137.
Abstract
Glial cell line-derived neurotrophic factor (GDNF) has a neuroprotective role in Purkinje cells of cerebellum, promoting the survival and the differentiation of these cells. Its signalling is mediated by a receptorial complex GFRalpha1/RET. In the brain of adult zebrafish (Danio rerio) we previously investigated GDNF expression and localization, but no data exist regarding GFRalpha1 and RET presence. Thus, the present study was designed to clarify the morphological relation between GDNF and its receptorial complex GFRalpha1/RET immunoreactivity in the cerebellum of adult zebrafish. The expression of gdnf, GFRalpha1 and ret genes was demonstrated in adult zebrafish cerebellum by a standard RT-PCR. The distribution of GDNF and its receptorial complex GFRalpha1/RET was examined by single and double immunocytochemical stainings. In the valvula and corpus cerebelli GDNF, GFRalpha1 and RET immunoreactivity was seen co-localized in Purkinje cells, identified morphologically and by using an antiserum against a specific marker for these cells, aldolase C enzyme. In the vestibulolateralis lobe, Purkinje neurons were lacking in both the eminentiae granulares and medial caudal lobe. These results demonstrated the expression of the GDNF receptorial complex in adult zebrafish cerebellum and suggest an autocrine mode of action of GDNF in Purkinje cells.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping