ZFIN ID: ZDB-PUB-090914-14
Early regulation of brain aromatase (cyp19a1b) by estrogen receptors during zebrafish development
Mouriec, K., Lareyre, J.J., Tong, S.K., Le Page, Y., Vaillant, C., Pellegrini, E., Pakdel, F., Chung, B.C., Kah, O., and Anglade, I.
Date: 2009
Source: Developmental dynamics : an official publication of the American Association of Anatomists   238(10): 2641-2651 (Journal)
Registered Authors: Chung, Bon-chu, Kah, Olivier, Lareyre, Jean-Jacques
Keywords: aromatase, estradiol, estrogen receptor, zebrafish, teleost, development, radial glial cell, neurosteroids, neurogenesis
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • Aromatase/genetics
  • Aromatase/metabolism*
  • Brain*/embryology
  • Brain*/enzymology
  • Embryo, Nonmammalian*/anatomy & histology
  • Embryo, Nonmammalian*/enzymology
  • Estradiol/analogs & derivatives
  • Estradiol/metabolism
  • Estrogen Antagonists/metabolism
  • Gene Expression Regulation, Developmental*
  • Protein Isoforms/genetics
  • Protein Isoforms/metabolism
  • Receptors, Estrogen/genetics
  • Receptors, Estrogen/metabolism*
  • Recombinant Fusion Proteins/genetics
  • Recombinant Fusion Proteins/metabolism
  • Zebrafish*/anatomy & histology
  • Zebrafish*/embryology
  • Zebrafish*/metabolism
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed: 19718764 Full text @ Dev. Dyn.
Early expression of estrogen receptors (esr) and their role in regulating early expression of cyp19a1b encoding brain aromatase were examined in the brain of zebrafish. Using in toto hybridization and quantitative reverse transcriptase-polymerase chain reaction (RT-PCR), a significant increase in the expression of esr1, esr2a, and esr2b was observed between 24 and 48 hours postfertilization (hpf). In toto hybridization demonstrated that esr2a and esr2b, but not esr1, are found in the hypothalamus. Using real-time RT-PCR, an increase in cyp19a1b mRNAs occurs between 24 and 48 hpf, indicating that expression of cyp19a1b is temporally correlated with that of esr. This increase is blocked by the pure anti-estrogen ICI182,780. Furthermore, E2 treatment of cyp19a1b-GFP (green fluorescent protein) transgenic embryos results in appearance of GFP expression in the brain as early as 25 hpf. These results indicate that basal expression of cyp19a1b expression in the brain of developing zebrafish most likely relies upon expression of esr that are fully functional before 25 hpf.