PUBLICATION
Somatic cell nuclear transfer in zebrafish
- Authors
- Siripattarapravat, K., Pinmee, B., Venta, P.J., Chang, C.C., and Cibelli, J.B.
- ID
- ZDB-PUB-090914-13
- Date
- 2009
- Source
- Nature Methods 6(10): 733-735 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Nuclear Transfer Techniques
- Animals
- Hybrid Cells/transplantation*
- Zebrafish/anatomy & histology*
- Zebrafish/genetics*
- Genetic Engineering/methods*
- PubMed
- 19718031 Full text @ Nat. Methods
Citation
Siripattarapravat, K., Pinmee, B., Venta, P.J., Chang, C.C., and Cibelli, J.B. (2009) Somatic cell nuclear transfer in zebrafish. Nature Methods. 6(10):733-735.
Abstract
We developed a method for somatic cell nuclear transfer in zebrafish using laser-ablated metaphase II eggs as recipients, the micropyle for transfer of the nucleus and an egg activation protocol after nuclear reconstruction. We produced clones from cells of both embryonic and adult origins, although the latter did not give rise to live adult clones.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping