PUBLICATION
Interleukin-17D in Atlantic salmon (Salmo salar): Molecular characterization, 3D modelling and promoter analysis
- Authors
- Kumari, J., Larsen, A.N., Bogwald, J., and Dalmo, R.A.
- ID
- ZDB-PUB-090914-10
- Date
- 2009
- Source
- Fish & shellfish immunology 27(5): 647-659 (Journal)
- Registered Authors
- Keywords
- IL-17D, Atlantic salmon, Cytokines, Promoter, mRNA expression
- MeSH Terms
-
- Models, Molecular
- Expressed Sequence Tags
- Molecular Sequence Data
- Random Amplified Polymorphic DNA Technique/veterinary
- Aeromonas salmonicida/growth & development
- Cloning, Molecular
- Phylogeny
- Sequence Alignment
- Interleukin-17/chemistry*
- Interleukin-17/genetics
- Interleukin-17/metabolism
- Interleukin-17/pharmacology
- Animals
- Amino Acid Sequence
- RNA, Messenger/biosynthesis
- RNA, Messenger/genetics
- Salmo salar/genetics
- Salmo salar/immunology*
- Promoter Regions, Genetic
- Base Sequence
- PubMed
- 19716422 Full text @ Fish Shellfish Immunol.
Citation
Kumari, J., Larsen, A.N., Bogwald, J., and Dalmo, R.A. (2009) Interleukin-17D in Atlantic salmon (Salmo salar): Molecular characterization, 3D modelling and promoter analysis. Fish & shellfish immunology. 27(5):647-659.
Abstract
IL-17 is a proinflammatory cytokine that plays an important role in the clearance of extracellular bacteria and contributes to the pathology of many autoimmune and allergic conditions. Much work on IL-17 has been done in humans and higher vertebrates while little work has been conducted in lower vertebrates including fish. In this study, we have cloned and characterized the full-length cDNA and genomic sequence of IL-17D from Atlantic salmon. The Atlantic salmon IL-17D (AsIL-17D) cDNA possessed an open reading frame of 621 bp encoding a putative protein of 206 aa with a predicted molecular weight of 23 kDa. The AsIL-17D gene has two exons and one intron showing the same (genome) organisation compared to zebrafish IL-17D. The encoded protein showed 97.6-48.8% identities to other IL-17D homologues, eight conserved cysteine residues were found within this group. Conserved residues believed to be important in receptor binding were also confirmed in salmon IL-17D by homology modelling. Phylogenetic analysis also confirmed the close relationship with other IL-17D homologues. Functional characterization of the 5' flanking region indicated that the region between -1552 and -150 contained sufficient elements for promoter activity. Tissue expression studies by real-time PCR showed a predominant expression of IL-17D transcript in gonads, skin, intestine, thymus of Atlantic salmon. The involvement of IL-17D during proinflammatory responses was demonstrated by investigating the time-dependent expression profile of IL-17D in head kidney and spleen following intraperitoneal injection of live Aeromonas salmonicida, LPS, and beta-glucan. This study provides further evidence for the existence of distinct homologue of IL-17D isoform in fish showing early expression induced by immunostimulants and bacterial infection that supports the fact that IL-17D is regulated by inflammatory processes in fish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping