PUBLICATION
Temporal and spatial patterns of gene expression in skeletal muscles in response to swim training in adult zebrafish (Danio rerio)
- Authors
- Lemoine, C.M., Craig, P.M., Dhekney, K., Kim, J.J., and McClelland, G.B.
- ID
- ZDB-PUB-090828-4
- Date
- 2010
- Source
- Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology 180(1): 151-160 (Journal)
- Registered Authors
- Keywords
- PGC-1, Mitochondrial enzymes, Gene expression, Fiber type, Oxidative metabolism, Exercise
- MeSH Terms
-
- Acyl-CoA Dehydrogenase/genetics
- Acyl-CoA Dehydrogenase/metabolism
- Animals
- Body Size
- Citrate (si)-Synthase/genetics
- Citrate (si)-Synthase/metabolism
- Gene Expression Regulation/physiology*
- Mitochondrial Proteins/genetics
- Mitochondrial Proteins/metabolism
- Muscle Fibers, Fast-Twitch/metabolism
- Muscle Fibers, Slow-Twitch/metabolism
- Muscle Proteins/genetics*
- Muscle Proteins/metabolism
- Muscle, Skeletal/anatomy & histology
- Muscle, Skeletal/metabolism*
- Organ Specificity
- PPAR-beta/genetics
- PPAR-beta/metabolism
- Physical Conditioning, Animal/physiology
- Physical Exertion/physiology*
- Protein Isoforms/genetics
- Protein Isoforms/metabolism
- RNA, Messenger/metabolism
- Swimming/physiology*
- Time Factors
- Transcription Factors/genetics
- Transcription Factors/metabolism
- Zebrafish/anatomy & histology
- Zebrafish/genetics
- Zebrafish/metabolism
- Zebrafish/physiology*
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- PubMed
- 19693513 Full text @ J. Comp. Physiol. B
Citation
Lemoine, C.M., Craig, P.M., Dhekney, K., Kim, J.J., and McClelland, G.B. (2010) Temporal and spatial patterns of gene expression in skeletal muscles in response to swim training in adult zebrafish (Danio rerio). Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology. 180(1):151-160.
Abstract
In adult zebrafish, 4 weeks of exercise training is known to induce an increase in mitochondrial enzymes such as citrate synthase (CS) when determined in mixed (red and white) muscle. However, this remodeling is not accompanied by changes in PGC-1alpha mRNA, a potent inducer of mitochondrial biogenesis in mammals. To further understand this response, we examined absolute and relative changes in red muscle area by histochemistry after 4 weeks of swim training. We also examined fiber-type specific responses in the expression of metabolic genes and putative regulators in red and white muscle of adult zebrafish at 1 and 8 weeks of training and in recovery from a single bout of exercise. Total red muscle area was unaltered after 4 weeks of training. The mRNA expression of CS was unaffected in red muscle, while it was increased in white muscle after 1 week of training and remained elevated at 8 weeks of training, suggesting an increase in oxidative capacity of this fiber type. In contrast, PGC-1alpha mRNA was elevated in both muscles only after 1 week of training. In both muscles, an acute bout of exercise rapidly (within 0-2 h post-exercise) induced PGC-1alpha mRNA and a delayed (24 h) increase in CS mRNA post-exercise. These results suggest complex temporal and spatial adaptive molecular responses to exercise in the skeletal muscles of zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping