PUBLICATION
Differential expression patterns and developmental roles of duplicated scinderin-like genes in zebrafish
- Authors
- Jia, S., Nakaya, N., and Piatigorsky, J.
- ID
- ZDB-PUB-090819-3
- Date
- 2009
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 238(10): 2633-2640 (Journal)
- Registered Authors
- Piatigorsky, Joram
- Keywords
- gene duplication, crystallin scinderin-like proteins, expression pattern, transgenic zebrafish, gene regulation
- MeSH Terms
-
- Animals
- Recombinant Fusion Proteins/genetics
- Recombinant Fusion Proteins/metabolism
- Sequence Alignment
- Molecular Sequence Data
- Amino Acid Sequence
- Oligonucleotides, Antisense/genetics
- Oligonucleotides, Antisense/metabolism
- Promoter Regions, Genetic
- Protein Isoforms/genetics
- Protein Isoforms/metabolism
- Embryo, Nonmammalian/anatomy & histology
- Embryo, Nonmammalian/physiology
- Zebrafish*/anatomy & histology
- Zebrafish*/embryology
- Zebrafish*/physiology
- Animals, Genetically Modified
- In Situ Hybridization
- Gene Expression Regulation, Developmental*
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- Gelsolin/genetics*
- Gelsolin/metabolism
- Body Patterning*
- PubMed
- 19681161 Full text @ Dev. Dyn.
Citation
Jia, S., Nakaya, N., and Piatigorsky, J. (2009) Differential expression patterns and developmental roles of duplicated scinderin-like genes in zebrafish. Developmental Dynamics : an official publication of the American Association of Anatomists. 238(10):2633-2640.
Abstract
Scinderin, the closest homologue of the actin-severing protein, gelsolin, has two similar paralogs (Scinla and Scinlb) in zebrafish. Scinla is abundant in the adult cornea; Scinlb comprises considerably less corneal protein. Here, we show that scinla is expressed in the nose, lens, brain, cornea and annular ligament of the iridocorneal angle; by contrast, scinlb is expressed in the hatching gland, floor plate, notochord, otic vesicle, brain, pharynx, cartilage, swim bladder and cornea. Activity of scinla and scinlb promoter fragments driving the EGFP reporter gene in transgenic zebrafish resembled scinla or scinlb expression. Previously, we showed that reduction of scinla by injection of antisense morpholino oligonucleotides ventralized embryos; here, specific reduction of scinlb expression led to subtle brain abnormalities associated with increased cell death, decreased shhb expression in the floor plate, and slightly reduced eye distance. Thus, scinla and scinlb have different expression patterns and developmental roles during zebrafish development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping