PUBLICATION

Differential expression patterns and developmental roles of duplicated scinderin-like genes in zebrafish

Authors

Jia, S., Nakaya, N., and Piatigorsky, J.

ID

ZDB-PUB-090819-3

Date

2009

Source

Developmental Dynamics : an official publication of the American Association of Anatomists 238(10): 2633-2640 (Journal)

Registered Authors

Piatigorsky, Joram

Keywords

gene duplication, crystallin scinderin-like proteins, expression pattern, transgenic zebrafish, gene regulation

MeSH Terms

Animals
Embryo, Nonmammalian/anatomy & histology
Embryo, Nonmammalian/physiology
Gelsolin/genetics*
Gelsolin/metabolism
Promoter Regions, Genetic
Zebrafish*/anatomy & histology
Zebrafish*/embryology
Zebrafish*/physiology
In Situ Hybridization
Recombinant Fusion Proteins/genetics
Recombinant Fusion Proteins/metabolism
Sequence Alignment
Oligonucleotides, Antisense/genetics
Oligonucleotides, Antisense/metabolism
Molecular Sequence Data
Protein Isoforms/genetics
Protein Isoforms/metabolism
Zebrafish Proteins/genetics*
Zebrafish Proteins/metabolism
Animals, Genetically Modified
Body Patterning*
Gene Expression Regulation, Developmental*
Amino Acid Sequence

(all 24)

PubMed

19681161 Full text @ Dev. Dyn.

Abstract

Scinderin, the closest homologue of the actin-severing protein, gelsolin, has two similar paralogs (Scinla and Scinlb) in zebrafish. Scinla is abundant in the adult cornea; Scinlb comprises considerably less corneal protein. Here, we show that scinla is expressed in the nose, lens, brain, cornea and annular ligament of the iridocorneal angle; by contrast, scinlb is expressed in the hatching gland, floor plate, notochord, otic vesicle, brain, pharynx, cartilage, swim bladder and cornea. Activity of scinla and scinlb promoter fragments driving the EGFP reporter gene in transgenic zebrafish resembled scinla or scinlb expression. Previously, we showed that reduction of scinla by injection of antisense morpholino oligonucleotides ventralized embryos; here, specific reduction of scinlb expression led to subtle brain abnormalities associated with increased cell death, decreased shhb expression in the floor plate, and slightly reduced eye distance. Thus, scinla and scinlb have different expression patterns and developmental roles during zebrafish development.

Genes / Markers

Figures

Show all Figures

Expression

Fish	Conditions	Stage	Anatomy	Assay	Figure
zf158Tg	standard conditions	Day 6	cartilage element	IFL	text only
zf158Tg	standard conditions	Prim-5	hatching gland	IFL	Fig. 4
zf158Tg	standard conditions	Protruding-mouth	notochord	IFL	Fig. 4
zf158Tg	standard conditions	Prim-5	notochord	IFL	Fig. 4
zf158Tg	standard conditions	Day 6	pharynx	IFL	text only
WT	standard conditions	Protruding-mouth	cornea	ISH	Fig. 2
WT	standard conditions	Day 6	cornea	ISH	Fig. 2
WT	standard conditions	Long-pec	corneal primordium	ISH	Fig. 2
WT	standard conditions	Protruding-mouth	eye	ISH	Fig. 2
WT	standard conditions	Prim-5	eye	ISH	Fig. 2

1 - 10 of 32

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Phenotype

Fish	Conditions	Stage	Phenotype	Figure
WT + MO2-scinlb	standard conditions	Prim-5	brain apoptotic, abnormal	Fig. 6
WT + MO2-scinlb	standard conditions	Prim-5	brain morphology, abnormal	Fig. 6
WT + MO2-scinlb	standard conditions	Prim-5	hindbrain opaque, abnormal	Fig. 6
WT + MO2-scinlb	standard conditions	Prim-5	midbrain opaque, abnormal	Fig. 6
WT + MO2-scinlb	standard conditions	Prim-5	neural tube apoptotic, abnormal	Fig. 6
WT + MO2-scinlb	standard conditions	Prim-5	brain apoptotic, abnormal	text only
WT + MO2-scinlb	standard conditions	Prim-5	brain morphology, abnormal	text only
WT + MO2-scinlb	standard conditions	Prim-5	hindbrain opaque, abnormal	text only
WT + MO2-scinlb	standard conditions	Prim-5	midbrain opaque, abnormal	text only
WT + MO2-scinlb	standard conditions	Prim-5	neural tube apoptotic, abnormal	text only

1 - 10 of 10

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Mutations / Transgenics

Allele	Construct	Type	Affected Genomic Region
zf158Tg	Tg(scinlb:EGFP)	Transgenic Insertion

Human Disease / Model

Sequence Targeting Reagents

Target	Reagent	Reagent Type
scinlb	MO1-scinlb	MRPHLNO
scinlb	MO2-scinlb	MRPHLNO

Fish

Fish
WT
WT + MO2-scinlb
zf158Tg

Antibodies

Orthology

Engineered Foreign Genes

Marker	Marker Type	Name
EGFP	EFG	EGFP

Mapping

Jia et al., 2009 ZDB-PUB-090819-3 PMID:19681161

Differential expression patterns and developmental roles of duplicated scinderin-like genes in zebrafish

Jia et al., 2009

ZDB-PUB-090819-3
PMID:19681161