PUBLICATION
Using in vivo zebrafish models to understand the biochemical basis of neutrophilic respiratory disease
- Authors
- Martin, J.S., and Renshaw, S.A.
- ID
- ZDB-PUB-090727-1
- Date
- 2009
- Source
- Biochemical Society transactions 37(Pt 4): 830-837 (Journal)
- Registered Authors
- Renshaw, Steve A.
- Keywords
- inflammation, interleukin, in vivo imaging, neutrophilic respiratory disease, zebrafish
- MeSH Terms
-
- Animals
- Disease Models, Animal*
- Inflammation/immunology
- Inflammation/pathology
- Neutrophils/immunology*
- Respiration Disorders/immunology*
- Respiration Disorders/pathology
- Respiration Disorders/physiopathology*
- Zebrafish
- PubMed
- 19614603 Full text @ Biochem Soc. Trans.
Citation
Martin, J.S., and Renshaw, S.A. (2009) Using in vivo zebrafish models to understand the biochemical basis of neutrophilic respiratory disease. Biochemical Society transactions. 37(Pt 4):830-837.
Abstract
Neutrophilic inflammation in the lung protects against infectious disease, and usually resolves spontaneously after removal of the inflammatory stimulus. However, much lung disease is caused by a failure of resolution of neutrophilic inflammation. Our laboratory is seeking an understanding of the biochemical basis of inflammation resolution, using the zebrafish model system. Zebrafish larvae are transparent, allowing visualization of GFP (green fluorescent protein)-labelled leucocytes during inflammation in vivo, and they can be readily manipulated by a range of forward and reverse genetic techniques. This combination of advantages makes zebrafish a powerful tool for the study of in vivo inflammatory processes. Using this model, we have visualized the process of inflammation resolution in vivo, and identified a role for apoptosis in this process. In addition, we have performed a forward genetic screen for mutants with defective resolution of inflammation, and reverse genetic experiments examining the influence of candidate genes on inflammation resolution. We have established a platform for screening for compounds with anti-inflammatory activity, which has yielded a number of interesting leads. Looking forward to succeed in the future, we are working at combining mutants, transgenes and pharmacological agents to dissect the biochemical basis of inflammation resolution, and to identify compounds that might be used to treat patients with respiratory disease.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping