PUBLICATION
Heteronuclear and Homonuclear Cu(2+) and Zn(2+) Complexes with Multihistidine Peptides Based on Zebrafish Prion-like Protein
- Authors
- Valensin, D., Szyrwiel, L., Camponeschi, F., Rowinska-Zyrek, M., Molteni, E., Jankowska, E., Szymanska, A., Gaggelli, E., Valensin, G., and Kozlowski, H.
- ID
- ZDB-PUB-090716-8
- Date
- 2009
- Source
- Inorganic chemistry 48(15): 7330-7340 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Histidine/chemistry
- Histidine/metabolism
- Prions/chemistry*
- Prions/metabolism
- Zinc/chemistry*
- Zinc/metabolism
- Circular Dichroism
- Copper/chemistry*
- Copper/metabolism
- Isomerism
- Nuclear Magnetic Resonance, Biomolecular
- Peptides/chemistry*
- Peptides/metabolism
- Zebrafish/metabolism*
- Protein Binding
- Animals
- PubMed
- 19586023 Full text @ Inorg. Chem.
Citation
Valensin, D., Szyrwiel, L., Camponeschi, F., Rowinska-Zyrek, M., Molteni, E., Jankowska, E., Szymanska, A., Gaggelli, E., Valensin, G., and Kozlowski, H. (2009) Heteronuclear and Homonuclear Cu(2+) and Zn(2+) Complexes with Multihistidine Peptides Based on Zebrafish Prion-like Protein. Inorganic chemistry. 48(15):7330-7340.
Abstract
The homeostasis of metal ions, especially copper and zinc, is a major factor that may influence the prion diseases and the biological function of prion protein (PrP). The His-rich regions are basic sites for metal binding and antioxidant activity of the PrP structures. Animal prion-like proteins contain also His-rich domains, and their coordination chemistry may provide better insight into the chemistry and biology of PrP structures and related diseases. Herein, we report an equilibrium study on heteronuclear Zn(2+)-Cu(2+) complexes with zrel-PrP fragments from zebrafish. Potentiometric, spectroscopic, and mass spectrometric methods showed that the binding of copper is much more effective than the binding of zinc. At physiological pH, both metals bind to the histidine imidazole N donors of the studied peptides.
Errata / Notes
Correction in: Inorg. Chem. 2009 Sept.;48(18):9042.Page 7330. The last name of the first author is misspelled in the original published version of the paper. It should be ?Valensin? instead of ?Vaslensin?.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping