PUBLICATION
An immune responsive complement factor D/adipsin and kallikrein-like serine protease (PoDAK) from the olive flounder Paralichthys olivaceus
- Authors
- Kong, H.J., Hong, G.E., Nam, B.H., Kim, Y.O., Kim, W.J., Lee, S.J., Lee, N.S., Do, J.W., Cho, H.K., Cheong, J., Lee, C.H., and Kim, K.K.
- ID
- ZDB-PUB-090716-16
- Date
- 2009
- Source
- Fish & shellfish immunology 27(3): 486-492 (Journal)
- Registered Authors
- Keywords
- Serine protease, Complement factor D/adipsin, Kallikrein, Olive flounder Paralichthys olivaceus, VHSV, Streptococcus iniae
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- Complement Factor D/chemistry
- Complement Factor D/genetics*
- Complement Factor D/immunology*
- Fish Diseases/enzymology
- Fish Diseases/immunology
- Flounder/classification
- Flounder/genetics*
- Flounder/immunology*
- Gene Expression Profiling
- Gene Expression Regulation*
- Kallikreins/chemistry
- Kallikreins/genetics*
- Kallikreins/immunology*
- Molecular Sequence Data
- Novirhabdovirus
- Phylogeny
- RNA, Messenger/immunology
- Rhabdoviridae Infections/enzymology
- Rhabdoviridae Infections/immunology
- Rhabdoviridae Infections/veterinary
- Sequence Alignment
- PubMed
- 19591942 Full text @ Fish Shellfish Immunol.
Citation
Kong, H.J., Hong, G.E., Nam, B.H., Kim, Y.O., Kim, W.J., Lee, S.J., Lee, N.S., Do, J.W., Cho, H.K., Cheong, J., Lee, C.H., and Kim, K.K. (2009) An immune responsive complement factor D/adipsin and kallikrein-like serine protease (PoDAK) from the olive flounder Paralichthys olivaceus. Fish & shellfish immunology. 27(3):486-492.
Abstract
The cDNA encoding of a complement factor D/adipsin and kallikrein-like serine protease, designated PoDAK, was isolated from the olive flounder Paralichthys olivaceus. PoDAK cDNA encodes a polypeptide with 277 amino acids containing conserved catalytic triad residues of serine proteases. The amino acid sequence of PoDAK showed high similarity to the kallikrein-like protein of medaka, mammalian adipsin/complement factor D and tissue kallikrein homolog, KT-14 of trout, complement factor D of zebrafish, and shared 31.6-36.8% homology with complement factor D/adipsin known from other species, including mammals. Phylogenetic analysis revealed that PoDAK clustered with the kallikrein-like protein of medaka and mammalian adipsin/complement factor D and tissue kallikrein homolog KT-14 of trout. The expression of PoDAK mRNA was high in the gills and heart, moderate in muscle, liver, intestine, stomach, kidney, and spleen of healthy flounder, and increased in the kidney, liver, and spleen of flounder challenged by the viral hemorrhagic septicemia virus (VHSV) or Streptococcus iniae. In situ hybridization confirmed that PoDAK mRNA is localized in the kidney and heart of individuals infected with VHSV. Further investigations are needed to clarify the function of PoDAK in vivo and in vitro.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping