PUBLICATION
CLOCK:BMAL-Independent Circadian Oscillation of Zebrafish Cryptochrome1a Gene
- Authors
- Miyamura, N., Hirayama, J., Sawanobori, K., Tamaru, T., Asaoka, Y., Honda, R., Yamamoto, T., Uno, H., Takamatsu, K., and Nishina, H.
- ID
- ZDB-PUB-090706-22
- Date
- 2009
- Source
- Biological & pharmaceutical bulletin 32(7): 1183-1187 (Journal)
- Registered Authors
- Hirayama, Jun
- Keywords
- circadian clock, cryptochrome, transcription
- MeSH Terms
-
- ARNTL Transcription Factors
- Animals
- Basic Helix-Loop-Helix Transcription Factors/genetics
- Basic Helix-Loop-Helix Transcription Factors/metabolism*
- Blotting, Western
- CLOCK Proteins
- Cells, Cultured
- Circadian Rhythm/genetics*
- Circadian Rhythm/physiology
- Light
- Protein Multimerization
- Reverse Transcriptase Polymerase Chain Reaction
- Trans-Activators/genetics
- Trans-Activators/metabolism*
- Transcription, Genetic
- Zebrafish/genetics*
- Zebrafish/physiology
- Zebrafish Proteins/genetics*
- PubMed
- 19571382 Full text @ Biol. Pharm. Bull.
Citation
Miyamura, N., Hirayama, J., Sawanobori, K., Tamaru, T., Asaoka, Y., Honda, R., Yamamoto, T., Uno, H., Takamatsu, K., and Nishina, H. (2009) CLOCK:BMAL-Independent Circadian Oscillation of Zebrafish Cryptochrome1a Gene. Biological & pharmaceutical bulletin. 32(7):1183-1187.
Abstract
In the vertebrate circadian feedback loop, CLOCK:BMAL heterodimers induce the expression of Cry genes. The CRY proteins in turn inhibit CLOCK:BMAL-mediated transcription closing the negative feedback loop. Four CRYs, which all inhibit CLOCK:BMAL-mediated transcription, exist in zebrafish. Although these zebrafish Crys (zCry1a, 1b, 2a, and 2b) show a circadian pattern of expression, previous studies have indicated that the circadian oscillation of zCry1a could be CLOCK:BMAL-independent. Here we show that abrogation of CLOCK:BMAL-dependent transcription in zebrafish cells by the dominant negative zCLOCK3-DeltaC does not affect the circadian oscillation of zCry1a. Moreover, we provide several lines of evidence indicating that the extracellular signal-regulated kinase (ERK) signaling cascade modulates the circadian expression of zCry1a gene in constant darkness. Taken together, our data strongly support the notion that circadian oscillation of zCry1a is CLOCK:BMAL-independent and further indicate that mechanisms involving non-canonical clock genes could contribute to the circadian expression of zCry1a gene in a cell autonomous manner.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping