PUBLICATION
Cystein-serine-rich nuclear protein 1, Axud1/Csrnp1, is essential for cephalic neural progenitor proliferation and survival in zebrafish
- Authors
- Feijoo, C.G., Sarrazin, A.F., Allende, M.L., and Glavic, A.
- ID
- ZDB-PUB-090629-18
- Date
- 2009
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 238(8): 2034-2043 (Journal)
- Registered Authors
- Allende, Miguel L., Feijoo, Carmen G., Sarrazin, Andrés
- Keywords
- axud1, brain growth, neural progenitors, proliferation, cell survival
- MeSH Terms
-
- Gene Targeting
- Cell Survival/genetics
- Cell Survival/physiology
- Phylogeny
- Embryonic Stem Cells/cytology
- Eye Proteins/genetics
- Eye Proteins/physiology
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish/physiology*
- Nerve Tissue Proteins/genetics
- Nerve Tissue Proteins/physiology
- Neurons/cytology
- Zebrafish Proteins/genetics
- Zebrafish Proteins/physiology*
- Homeodomain Proteins/genetics
- Homeodomain Proteins/physiology
- Humans
- Animals
- DNA, Complementary/genetics
- Molecular Sequence Data
- Sequence Homology, Amino Acid
- Amino Acid Sequence
- Cell Proliferation
- Mice
- Hedgehog Proteins/genetics
- Hedgehog Proteins/physiology
- Body Patterning/genetics
- Body Patterning/physiology
- Base Sequence
- Brain/cytology
- Brain/embryology*
- Nuclear Proteins/genetics
- Nuclear Proteins/physiology*
- Gene Expression Regulation, Developmental
- PubMed
- 19544579 Full text @ Dev. Dyn.
Citation
Feijoo, C.G., Sarrazin, A.F., Allende, M.L., and Glavic, A. (2009) Cystein-serine-rich nuclear protein 1, Axud1/Csrnp1, is essential for cephalic neural progenitor proliferation and survival in zebrafish. Developmental Dynamics : an official publication of the American Association of Anatomists. 238(8):2034-2043.
Abstract
The CSRNP (cystein-serine-rich nuclear protein) family has been conserved from Drosophila to human. Although knockout mice for each of the mammalian proteins have been generated, their function during vertebrate development has remained elusive. As an alternative to obtain insights on CSRNP's role in development, we have analysed the expression pattern and function of one member of this family, axud1, during zebrafish development. Our expression analysis indicates that axud1 is expressed from cleavage to larval stages in a dynamic pattern, becoming restricted after gastrulation to anterior regions of the developing neuraxis and later on concentrated predominantly in proliferating domains of the brain. Knockdown analysis using antisense morpholinos shows that reducing Axud1 levels impairs neural progenitor cell proliferation and survival, revealing an essential function of this gene for the growth of cephalic derivatives. The brain growth phenotypes elicited by decreasing Axud1 expression are specific and independent of anterior-posterior patterning events, initial establishment of neural progenitors, or neural differentiation occurring in this tissue. However, Axud1 is necessary for six3.1 expression and is positively regulated by sonic hedgehog. Phylogenetic examination shows that axud1 is likely to be the ortholog of the only member of this family present in Drosophila, as well as to the previously described mouse CSRNP1 and to human AXUD1 (Axin upregulated-1). Thus, we provide evidence as to the role of axud1 in brain growth in vertebrates.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping