PUBLICATION
Definition of three somatic adult cell nuclear transplant methods in zebrafish (Danio rerio): before, during and after egg activation by sperm fertilization
- Authors
- Pérez-Camps, M., Cardona-Costa, J., Francisco-Simao, M., and García-Ximénez, F.
- ID
- ZDB-PUB-090616-57
- Date
- 2010
- Source
- Zygote (Cambridge, England) 18(1): 33-39 (Journal)
- Registered Authors
- Cardona-Costa, Jose
- Keywords
- Egg activation, Fertilization, Microinjection, Nuclear transplant, Zebrafish
- MeSH Terms
-
- Aging*
- Animals
- Cells, Cultured
- Female
- Fertilization*
- Male
- Nuclear Transfer Techniques*
- Ovum/physiology*
- Spermatozoa/physiology*
- Zebrafish/physiology*
- PubMed
- 19519982 Full text @ Zygote
Citation
Pérez-Camps, M., Cardona-Costa, J., Francisco-Simao, M., and García-Ximénez, F. (2010) Definition of three somatic adult cell nuclear transplant methods in zebrafish (Danio rerio): before, during and after egg activation by sperm fertilization. Zygote (Cambridge, England). 18(1):33-39.
Abstract
Zebrafish somatic nuclear transplant has only been attempted using preactivated eggs. In this work, three methods to carry out the nuclear transplant using adult cells before, during and after the egg activation/fertilization were developed in zebrafish with the aim to be used in reprogramming studies. The donor nucleus from somatic adult cells was inserted: (method A) in the central region of the egg and subsequently fertilized; (method B) in the incipient animal pole at the same time that the egg was fertilized; and (method C) in the completely defined animal pole after fertilization. Larval and adult specimens were obtained using the three methods. Technical aspects related to temperature conditions, media required, egg activation/fertilization, post-ovulatory time of the transplant, egg aging, place of the donor nucleus injection in each methodology are presented. In conclusion, the technical approach developed in this work can be used in reprogramming studies.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping