PUBLICATION
Early lens development in the zebrafish: A three-dimensional time-lapse analysis
- Authors
- Greiling, T.M., and Clark, J.I.
- ID
- ZDB-PUB-090616-40
- Date
- 2009
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 238(9): 2254-2265 (Journal)
- Registered Authors
- Keywords
- lens, development, delamination, lens mass, zebrafish, 3D, imaging
- MeSH Terms
-
- Animals
- Gene Expression Regulation, Developmental
- Lens, Crystalline/embryology*
- Microscopy, Fluorescence, Multiphoton/methods*
- Zebrafish/embryology*
- PubMed
- 19504455 Full text @ Dev. Dyn.
Citation
Greiling, T.M., and Clark, J.I. (2009) Early lens development in the zebrafish: A three-dimensional time-lapse analysis. Developmental Dynamics : an official publication of the American Association of Anatomists. 238(9):2254-2265.
Abstract
In vivo, high-resolution, time-lapse imaging characterized lens development in the zebrafish from 16 to 96 hr postfertilization (hpf). In zebrafish, the lens placode appeared in the head ectoderm, similar to mammals. Delamination of the surface ectoderm resulted in the formation of the lens mass, which progressed to a solid sphere of cells separating from the developing cornea at approximately 24 hpf. A lens vesicle was not observed and apoptosis was not a major factor in separation of the lens from the future cornea. Differentiation of primary fibers began in the lens mass followed by formation of the anterior epithelium after delamination was complete. Secondary fibers differentiated from elongating epithelial cells near the posterior pole. Quantification characterized three stages of lens growth. The study confirmed the advantages of live-cell imaging for three-dimensional quantitative structural characterization of the mechanism(s) responsible for cell differentiation in formation of a transparent, symmetric, and refractile lens.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping