PUBLICATION
Identification of phagocytic cells, NK-like cytotoxic cell activity and the production of cellular exudates in the coelomic cavity of adult zebrafish
- Authors
- Moss, L.D., Monette, M.M., Jaso-Friedmann, L., Leary, J.H. 3rd, Dougan, S.T., Krunkosky, T., and Evans, D.L.
- ID
- ZDB-PUB-090601-16
- Date
- 2009
- Source
- Developmental and comparative immunology 33(10): 1077-1087 (Journal)
- Registered Authors
- Dougan, Scott T., Evans, Donald L., Jaso-Friedmann, Liliana
- Keywords
- Zebrafish, Coelomic cavity, Leukocytes, Phagocytosis, Nonspecific cytotoxic cells, Redirected lysis, Innate immunity, Adherent cells
- MeSH Terms
-
- Abdominal Cavity
- Animals
- Cell Line, Tumor
- Cells, Cultured
- Cytotoxicity Tests, Immunologic
- Cytotoxicity, Immunologic/immunology*
- Escherichia coli/immunology
- Exudates and Transudates/metabolism*
- Female
- Flow Cytometry
- Gene Expression
- HL-60 Cells
- Humans
- K562 Cells
- Killer Cells, Natural/cytology
- Killer Cells, Natural/immunology
- Killer Cells, Natural/metabolism
- Leukocytes/cytology
- Leukocytes/immunology
- Leukocytes/metabolism
- Microscopy, Confocal
- Microscopy, Electron
- Phagocytes/cytology
- Phagocytes/immunology*
- Phagocytes/ultrastructure
- Phagocytosis/immunology
- Receptors, Antigen/genetics
- Receptors, Antigen/metabolism
- Reverse Transcriptase Polymerase Chain Reaction
- Zebrafish/immunology*
- Zebrafish/metabolism
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 19477195 Full text @ Dev. Comp. Immunol.
Citation
Moss, L.D., Monette, M.M., Jaso-Friedmann, L., Leary, J.H. 3rd, Dougan, S.T., Krunkosky, T., and Evans, D.L. (2009) Identification of phagocytic cells, NK-like cytotoxic cell activity and the production of cellular exudates in the coelomic cavity of adult zebrafish. Developmental and comparative immunology. 33(10):1077-1087.
Abstract
Coelomic cavity (CC) cells of mature zebrafish harvested by lavage with media or trypsin-EDTA contained 0.80-1.20x10(5) and 2.0-3.5x10(5) cells, respectively. Media lavage was composed of granulocytes (60-80%), lymphocytes (10-20%), and NCC (4-10%). Granulocytes had large electron dense cytoplasmic paracrystalline granules and a segmented nucleus; they expressed plastin-1, myeloid specific peroxidase and MCSF mRNA; and they were NCAMP-1(+). Lymphocytes had B- and T-cell specific mRNA and were NCAMP-1(-) and NCCRP-1(-). NCC were 3mu, NCAMP-1(+) and NCCRP-1(+) and did not express B- and T-cell specific mRNA. Additionally, trypsin lavage contained monocytes (marginated chromatin, low nuclear:cytoplasm ratio, sparse cytosolic granules) and macrophages (nonsegmented nuclei, no margination of chromatin, abundant electron dense granules). E. coli injected into the CC were phagocytosed in a dose and time dependent fashion by granulocytes, monocytes and macrophages. NCC lysed mammalian target cells and NCAMP-1 expressing hybridoma cells in redirected lysis assays.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping