PUBLICATION

Identification of phagocytic cells, NK-like cytotoxic cell activity and the production of cellular exudates in the coelomic cavity of adult zebrafish

Authors
Moss, L.D., Monette, M.M., Jaso-Friedmann, L., Leary, J.H. 3rd, Dougan, S.T., Krunkosky, T., and Evans, D.L.
ID
ZDB-PUB-090601-16
Date
2009
Source
Developmental and comparative immunology   33(10): 1077-1087 (Journal)
Registered Authors
Dougan, Scott T., Evans, Donald L., Jaso-Friedmann, Liliana
Keywords
Zebrafish, Coelomic cavity, Leukocytes, Phagocytosis, Nonspecific cytotoxic cells, Redirected lysis, Innate immunity, Adherent cells
MeSH Terms
  • Abdominal Cavity
  • Animals
  • Cell Line, Tumor
  • Cells, Cultured
  • Cytotoxicity Tests, Immunologic
  • Cytotoxicity, Immunologic/immunology*
  • Escherichia coli/immunology
  • Exudates and Transudates/metabolism*
  • Female
  • Flow Cytometry
  • Gene Expression
  • HL-60 Cells
  • Humans
  • K562 Cells
  • Killer Cells, Natural/cytology
  • Killer Cells, Natural/immunology
  • Killer Cells, Natural/metabolism
  • Leukocytes/cytology
  • Leukocytes/immunology
  • Leukocytes/metabolism
  • Microscopy, Confocal
  • Microscopy, Electron
  • Phagocytes/cytology
  • Phagocytes/immunology*
  • Phagocytes/ultrastructure
  • Phagocytosis/immunology
  • Receptors, Antigen/genetics
  • Receptors, Antigen/metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Zebrafish/immunology*
  • Zebrafish/metabolism
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
PubMed
19477195 Full text @ Dev. Comp. Immunol.
Abstract
Coelomic cavity (CC) cells of mature zebrafish harvested by lavage with media or trypsin-EDTA contained 0.80-1.20x10(5) and 2.0-3.5x10(5) cells, respectively. Media lavage was composed of granulocytes (60-80%), lymphocytes (10-20%), and NCC (4-10%). Granulocytes had large electron dense cytoplasmic paracrystalline granules and a segmented nucleus; they expressed plastin-1, myeloid specific peroxidase and MCSF mRNA; and they were NCAMP-1(+). Lymphocytes had B- and T-cell specific mRNA and were NCAMP-1(-) and NCCRP-1(-). NCC were 3mu, NCAMP-1(+) and NCCRP-1(+) and did not express B- and T-cell specific mRNA. Additionally, trypsin lavage contained monocytes (marginated chromatin, low nuclear:cytoplasm ratio, sparse cytosolic granules) and macrophages (nonsegmented nuclei, no margination of chromatin, abundant electron dense granules). E. coli injected into the CC were phagocytosed in a dose and time dependent fashion by granulocytes, monocytes and macrophages. NCC lysed mammalian target cells and NCAMP-1 expressing hybridoma cells in redirected lysis assays.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping