PUBLICATION
Waterborne exposure to fluorotelomer alcohol 6:2 FTOH alters plasma sex hormone and gene transcription in the hypothalamic-pituitary-gonadal (HPG) axis of zebrafish
- Authors
- Liu, C., Yu, L., Deng, J., Lam, P.K., Wu, R.S., and Zhou, B.
- ID
- ZDB-PUB-090518-23
- Date
- 2009
- Source
- Aquatic toxicology (Amsterdam, Netherlands) 93(2-3): 131-137 (Journal)
- Registered Authors
- Yu, Liqun
- Keywords
- Fluorotelomer alcohols, Hypothalamic–pituitary–gonadal (HPG) axis, Gene transcription, Sex hormone, Endocrine disruption
- MeSH Terms
-
- Animals
- Endocrine Disruptors/toxicity*
- Environmental Exposure
- Estradiol/blood
- Estrogens/toxicity*
- Female
- Fluorocarbons/toxicity*
- Gene Expression/drug effects
- Gonadal Steroid Hormones/blood*
- Hypothalamo-Hypophyseal System/metabolism
- Liver/metabolism
- Male
- Testosterone/blood
- Transcription, Genetic/drug effects*
- Water Pollutants, Chemical/toxicity*
- Zebrafish/blood
- Zebrafish/genetics
- Zebrafish/metabolism*
- PubMed
- 19443055 Full text @ Aquat. Toxicol.
Citation
Liu, C., Yu, L., Deng, J., Lam, P.K., Wu, R.S., and Zhou, B. (2009) Waterborne exposure to fluorotelomer alcohol 6:2 FTOH alters plasma sex hormone and gene transcription in the hypothalamic-pituitary-gonadal (HPG) axis of zebrafish. Aquatic toxicology (Amsterdam, Netherlands). 93(2-3):131-137.
Abstract
Fluorotelomer alcohols (FTOHs) have shown estrogenic activity in vitro and in vivo, but the mechanism of this activity is not known. In this study, 18-week-old zebrafish (Danio rerio) were exposed to 0, 0.03, 0.3 and 3.0mg/l 1H,1H,2H,2H-perfluorooctan-1-ol (6:2 FTOH) for 7 days, and the effects on plasma sex hormone levels were measured followed by use of real-time PCR to examine selected gene expression in hypothalamic-pituitary-gonadal (HPG) axis and liver. Exposure to 6:2 FTOH significantly increased plasma estradiol (E2) and testosterone (T) levels in both males and females. Furthermore, the ratio of T/E2 was reduced in females while increased in males. In females, the increase of E2 was accompanied by up-regulated hepatic estrogenic receptor alpha (ERalpha) and vitellogenin (VTG1 and VTG3) expression. In males, the elevation of the T level is consistent with the up-regulation of cytochrome P450 c17alpha-hydroxylase, 17, 20-lase (CYP17) and the down-regulation of cytochrome P450 aromatase A (CYP19A). The present study demonstrated that waterborne exposure to 6:2 FTOH alter plasma sex hormone levels and the ratio of T/E2, as well as the transcriptional profiles of some genes in the HPG axis and liver. The results suggested that FTOHs may disturb fish reproduction through endocrine disrupted activity.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping