PUBLICATION

Alternative splicing, phylogenetic analysis, and craniofacial expression of zebrafish tbx22

Authors

Jezewski, P.A., Fang, P.K., Payne-Ferreira, T.L., and Yelick, P.C.

ID

ZDB-PUB-090511-10

Date

2009

Source

Developmental Dynamics : an official publication of the American Association of Anatomists 238(6): 1605-1612 (Journal)

Registered Authors

Fang, Pingke, Jezewski, Peter, Payne-Ferreira, Tracie, Yelick, Pamela C.

Keywords

zebrafish, craniofacial development, tbx22

MeSH Terms

Zebrafish/anatomy & histology
Zebrafish/embryology
Zebrafish/genetics*
Zebrafish/physiology
Animals
Phylogeny
Skull/anatomy & histology
Skull/embryology*
Skull/physiology
Facial Bones/anatomy & histology
Facial Bones/embryology*
Facial Bones/physiology
Craniofacial Abnormalities/genetics
Zebrafish Proteins/chemistry
Zebrafish Proteins/genetics*
Zebrafish Proteins/metabolism*
Sequence Alignment
Gene Expression Regulation, Developmental
Protein Isoforms/genetics
Protein Isoforms/metabolism
Molecular Sequence Data
Amino Acid Sequence
Body Patterning/genetics
Base Sequence
Humans
Alternative Splicing*
T-Box Domain Proteins/chemistry
T-Box Domain Proteins/classification
T-Box Domain Proteins/genetics*
T-Box Domain Proteins/metabolism*

(all 30)

PubMed

19418442 Full text @ Dev. Dyn.

Abstract

Mutations in human TBX22 cause X-linked cleft palate with ankyloglossia syndrome (CPX; OMIM 303400). Since the secondary palate was an adaptation to breathing on land, we characterized zebrafish tbx22 to study molecular mechanisms regulating early vertebrate craniofacial patterning. Rapid Amplification of cDNA Ends (RACE) analyses revealed two zebrafish tbx22 splice isoforms, tbx22-1 and tbx22-2, encoding proteins of 444 and 400 amino acids, respectively. tbx22-1 resembles canonical Tbx22 orthologs, while tbx22-2 lacks conserved N-terminal sequence. Developmental RT-PCR revealed that tbx22-1 is maternally and zygotically expressed, while tbx22-2 is expressed zygotically. WISH analyses revealed strong tbx22 mRNA expression in ectomesenchyme underlying the stomodeum, a bilaminar epithelial structure demarcating early mouth formation, and in early presumptive jaw joints. Zebrafish tbx22 expression mirrored some aspects of mammalian Tbx22, consistent with roles in early vertebrate face patterning. These studies identify an early transcription factor governing vertebrate facial development, which may underlie common craniofacial birth disorders.

Genes / Markers

Figures

Show all Figures

Expression

Gene	Fish	Conditions	Stage	Anatomy	Assay	Figure
nkx3-2	WT	standard conditions	Long-pec	mandibular arch skeleton	ISH	Fig. 6
tbx22	WT	standard conditions	Long-pec	mandibular arch skeleton	ISH	Fig. 6
tbx22	WT	standard conditions	Prim-5	paraxial mesoderm	ISH	Fig. 6
tbx22	WT	standard conditions	Prim-25	stomodeum	ISH	Fig. 6
tbx22	WT	standard conditions	Day 5	whole organism	RTPCR	Fig. 5
tbx22	WT	standard conditions	Prim-25	whole organism	RTPCR	Fig. 5
tbx22	WT	standard conditions	Days 30-44	whole organism	RTPCR	Fig. 5
tbx22	WT	standard conditions	Adult	whole organism	RTPCR	Fig. 5
tbx22	WT	standard conditions	2-cell	whole organism	RTPCR	Fig. 5
tbx22	WT	standard conditions	Germ-ring	whole organism	RTPCR	Fig. 5

1 - 10 of 11

Show

Phenotype

Mutations / Transgenics

Human Disease / Model

Sequence Targeting Reagents

Fish

Fish
WT

Antibodies

Orthology

Gene	Orthology
tbx1
tbx15
tbx18
tbx20
tbx22

Engineered Foreign Genes

Mapping

Jezewski et al., 2009 ZDB-PUB-090511-10 PMID:19418442

Alternative splicing, phylogenetic analysis, and craniofacial expression of zebrafish tbx22

Jezewski et al., 2009

ZDB-PUB-090511-10
PMID:19418442