PUBLICATION
Line scan fluorescence correlation spectroscopy for three-dimensional microfluidic flow velocity measurements
- Authors
- Pan, X., Shi, X., Korzh, V., Yu, H., and Wohland, T.
- ID
- ZDB-PUB-090505-8
- Date
- 2009
- Source
- Journal of Biomedical Optics 14(2): 024049 (Journal)
- Registered Authors
- Korzh, Vladimir
- Keywords
- none
- MeSH Terms
-
- Algorithms*
- Image Interpretation, Computer-Assisted/methods*
- Microfluidics/methods*
- Reproducibility of Results
- Sensitivity and Specificity
- Spectrometry, Fluorescence/methods*
- PubMed
- 19405777 Full text @ J. Biomed. Opt.
Citation
Pan, X., Shi, X., Korzh, V., Yu, H., and Wohland, T. (2009) Line scan fluorescence correlation spectroscopy for three-dimensional microfluidic flow velocity measurements. Journal of Biomedical Optics. 14(2):024049.
Abstract
The flow direction of microfluidics in biological applications is not limited to two dimensions, but often extends to three dimensions. Currently there are optical methods available for the measurement of 3-D microfluidic flow vectors, but with low spatial resolution. Line scan fluorescence correlation spectroscopy (FCS) was proposed to determine flow directions in 2-D within microchannels and small blood vessels in our previous work. Importantly, its spatial resolution was demonstrated to be as good as 0.5 mum. In this work, we extend line scan FCS to the third dimension for the characterization of 3-D flow velocity vectors. The spatial resolution is close to the diffraction limit using a scan length of 0.5 mum in all three dimensions. The feasibility of line scan FCS for 3-D microfluidic flow is verified by measurements in microchannels and small blood vessels of zebrafish embryos.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
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