Optimization of volumetric computed tomography for skeletal analysis of model genetic organisms
- Vasquez, S.X., Hansen, M.S., Bahadur, A.N., Hockin, M.F., Kindlmann, G.L., Nevell, L., Wu, I.Q., Grunwald, D.J., Weinstein, D.M., Jones, G.M., Johnson, C.R., Vandeberg, J.L., Capecchi, M.R., and Keller, C.
- The Anatomical record 291(5): 475-487 (Journal)
- Registered Authors
- Grunwald, David
- volumetric x-ray computed tomography, microCT, phenomics, fetus, phenotyping, embryogenesis, imaging, mouse
- MeSH Terms
- Animals, Newborn
- Chick Embryo
- Chiroptera/anatomy & histology
- Ducks/anatomy & histology
- Lemur/anatomy & histology
- Models, Animal*
- Tomography, X-Ray Computed*
- Xenopus laevis/anatomy & histology
- Zebrafish/anatomy & histology
- 18286615 Full text @ Anat. Rec.
Vasquez, S.X., Hansen, M.S., Bahadur, A.N., Hockin, M.F., Kindlmann, G.L., Nevell, L., Wu, I.Q., Grunwald, D.J., Weinstein, D.M., Jones, G.M., Johnson, C.R., Vandeberg, J.L., Capecchi, M.R., and Keller, C. (2008) Optimization of volumetric computed tomography for skeletal analysis of model genetic organisms. The Anatomical record. 291(5):475-487.
Forward and reverse genetics now allow researchers to understand embryonic and postnatal gene function in a broad range of species. Although some genetic mutations cause obvious morphological change, other mutations can be more subtle and, without adequate observation and quantification, might be overlooked. For the increasing number of genetic model organisms examined by the growing field of phenomics, standardized but sensitive methods for quantitative analysis need to be incorporated into routine practice to effectively acquire and analyze ever-increasing quantities of phenotypic data. In this study, we present platform-independent parameters for the use of microscopic x-ray computed tomography (microCT) for phenotyping species-specific skeletal morphology of a variety of different genetic model organisms. We show that microCT is suitable for phenotypic characterization for prenatal and postnatal specimens across multiple species.
Genes / Markers
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Engineered Foreign Genes
Errata and Notes