PUBLICATION
Tissue micromanipulation in zebrafish embryos
- Authors
- Picker, A., Roellig, D., Pourquié, O., Oates, A.C., and Brand, M.
- ID
- ZDB-PUB-090424-4
- Date
- 2009
- Source
- Methods in molecular biology (Clifton, N.J.) 546: 153-172 (Chapter)
- Registered Authors
- Brand, Michael, Oates, Andrew, Roellig, Daniela
- Keywords
- Zebrafish, Danio rerio, Transplantation, Bead implantation, Presomitic mesoderm, Optic vesicle, Dissection, Micromanipulation
- MeSH Terms
-
- Animals
- Chimerism
- Embryo Culture Techniques
- Embryo, Nonmammalian/transplantation*
- Eye*/embryology
- Eye*/transplantation
- Microdissection/instrumentation
- Microdissection/methods
- Micromanipulation/instrumentation
- Micromanipulation/methods*
- Specimen Handling/instrumentation
- Specimen Handling/methods
- Zebrafish*
- PubMed
- 19378104 Full text @ Meth. Mol. Biol.
Citation
Picker, A., Roellig, D., Pourquié, O., Oates, A.C., and Brand, M. (2009) Tissue micromanipulation in zebrafish embryos. Methods in molecular biology (Clifton, N.J.). 546:153-172.
Abstract
Although a common approach in large vertebrate embryos such as chick or frog, manipulation at the tissue level is only rarely applied to zebrafish embryos. Despite its relatively small size, the zebrafish embryo can be readily used for micromanipulations such as tissue and organ primordium transplantation, explantation, and microbead implantation, to study inductive tissue interactions and tissue autonomy of pleiotropic, mutant phenotypes or to isolate tissue for organotypic and primary cell culture or RNA isolation. Since this requires special handling techniques, tools, and tricks, which are rarely published and thus difficult to apply without hands-on demonstration, this article provides detailed instructions and protocols on tissue micromanipulation. The goal is to introduce a broader scientific audience to these surgical techniques, which can be applied to a wide range of questions and used as a starting point for many downstream applications in the genetically tractable zebrafish embryo.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping