PUBLICATION
Expression regulation of Na+-K+-ATPase {alpha}1-subunit subtypes in zebrafish gill ionocytes
- Authors
- Liao, B.K., Chen, R.D., and Hwang, P.P.
- ID
- ZDB-PUB-090424-30
- Date
- 2009
- Source
- American journal of physiology. Regulatory, integrative and comparative physiology 296(6): R1897-R1906 (Journal)
- Registered Authors
- Hwang, Pung Pung
- Keywords
- none
- MeSH Terms
-
- Animals
- Calcium/metabolism
- Calcium Channels/metabolism
- Chlorides/metabolism
- Gene Expression Regulation, Enzymologic
- Gills/cytology
- Gills/enzymology*
- Phylogeny
- Protein Subunits
- Proton-Translocating ATPases/metabolism
- RNA, Messenger/metabolism
- Sodium/metabolism
- Sodium Chloride Symporters/metabolism
- Sodium-Hydrogen Exchangers/metabolism
- Sodium-Potassium-Exchanging ATPase/genetics
- Sodium-Potassium-Exchanging ATPase/metabolism*
- Water-Electrolyte Balance
- Zebrafish
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 19386990 Full text @ Am. J. Physiol. Regul. Integr. Comp. Physiol.
Citation
Liao, B.K., Chen, R.D., and Hwang, P.P. (2009) Expression regulation of Na+-K+-ATPase {alpha}1-subunit subtypes in zebrafish gill ionocytes. American journal of physiology. Regulatory, integrative and comparative physiology. 296(6):R1897-R1906.
Abstract
In zebrafish (Danio rerio), 6 distinct Na-K-ATPase (NKA) alpha1 subunit genes have been identified, and 4 of them, zATP1a1a.1, zATP1a1a.2, zATP1a1a.4, and zATP1a1a.5, are expressed in embryonic skin, where different types of ionocytes appear. The present study attempted to test a hypothesis of whether these NKA alpha1 paralogues are specifically expressed and function in respective ionocytes. Double fluorescence in situ hybridization analysis demonstrated the specific expression of zATP1a1a.1, zATP1a1a.2, and zATP1a1a.5 in NKA-rich (NaR) cells, Na(+)-Cl(-) cotransporter (NCC)-expressing cells, and H(+)-ATPase-rich (HR) cells, respectively, based the colocalization of the 3 NKA alpha1 genes with marker genes of the respective ionocytes (epithelial Ca(2+) channel in NaR cells; NCC in NCC cells; and H(+)-ATPase and Na(+)/H(+) exchanger 3b in HR cells). The mRNA expression (by real-time PCR) of zATP1a1a.1, zATP1a1a.2, and zATP1a1a.5 were respectively upregulated by low-Ca(2+), low-Cl(-), and low-Na(+) fresh water, which had previously been reported to stimulate uptake functions of Ca(2+), Cl(-), and Na(+). However, zATP1a1a.4 was not colocalized with any of the 3 types of ionocytes, nor did its mRNA respond to the ambient ions examined. Taken together, zATP1a1a.1, zATP1a1a.2, and zATP1a1a.5 may provide driving force for Na(+) coupled cotransporter activity specifically in NaR, NCC, and HR cells, respectively. Key words: mitochondrion-rich cells, ion regulation, osmoregulation, fish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping