PUBLICATION
Focal electroporation in zebrafish embryos and larvae
- Authors
- Tawk, M., Bianco, I.H., and Clarke, J.D.
- ID
- ZDB-PUB-090424-3
- Date
- 2009
- Source
- Methods in molecular biology (Clifton, N.J.) 546: 145-151 (Chapter)
- Registered Authors
- Bianco, Isaac, Clarke, Jon, Tawk, Marcel
- Keywords
- Zebrafish, Electroporation, DNA, RNA, Fluorescent dextran, Morpholino
- MeSH Terms
-
- Larva/cytology
- Larva/drug effects
- Fluorescent Dyes/pharmacology
- Embryo, Nonmammalian/cytology
- Embryo, Nonmammalian/drug effects
- Animals
- Zebrafish/embryology*
- Zebrafish/genetics*
- Zebrafish/metabolism
- Animals, Genetically Modified/embryology
- Animals, Genetically Modified/genetics
- Animals, Genetically Modified/metabolism
- Time Factors
- Electroporation/methods*
- Microscopy
- DNA/genetics
- DNA/metabolism
- Developmental Biology/methods*
- Rhodamines/pharmacology
- RNA/genetics
- RNA/metabolism
- PubMed
- 19378103 Full text @ Meth. Mol. Biol.
Citation
Tawk, M., Bianco, I.H., and Clarke, J.D. (2009) Focal electroporation in zebrafish embryos and larvae. Methods in molecular biology (Clifton, N.J.). 546:145-151.
Abstract
A method is described that allows the introduction by electroporation of either small dyes or larger RNA, DNA, or morpholino constructs into single cells or small groups of cells in zebrafish embryos or larvae. The dye or construct is delivered to cells via a patch-like microelectrode that also delivers the electroporation stimulus train. This technique allows the experimenter to target cells of their choice at a particular time of development and at a particular location in the embryo, and is useful for fate mapping, analysing neuronal organisation, ectopic expression and gene knockdown experiments.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping