PUBLICATION

Focal electroporation in zebrafish embryos and larvae

Authors
Tawk, M., Bianco, I.H., and Clarke, J.D.
ID
ZDB-PUB-090424-3
Date
2009
Source
Methods in molecular biology (Clifton, N.J.)   546: 145-151 (Chapter)
Registered Authors
Bianco, Isaac, Clarke, Jon, Tawk, Marcel
Keywords
Zebrafish, Electroporation, DNA, RNA, Fluorescent dextran, Morpholino
MeSH Terms
  • Animals
  • Animals, Genetically Modified/embryology
  • Animals, Genetically Modified/genetics
  • Animals, Genetically Modified/metabolism
  • DNA/genetics
  • DNA/metabolism
  • Developmental Biology/methods*
  • Electroporation/methods*
  • Embryo, Nonmammalian/cytology
  • Embryo, Nonmammalian/drug effects
  • Fluorescent Dyes/pharmacology
  • Larva/cytology
  • Larva/drug effects
  • Microscopy
  • RNA/genetics
  • RNA/metabolism
  • Rhodamines/pharmacology
  • Time Factors
  • Zebrafish/embryology*
  • Zebrafish/genetics*
  • Zebrafish/metabolism
PubMed
19378103 Full text @ Meth. Mol. Biol.
Abstract
A method is described that allows the introduction by electroporation of either small dyes or larger RNA, DNA, or morpholino constructs into single cells or small groups of cells in zebrafish embryos or larvae. The dye or construct is delivered to cells via a patch-like microelectrode that also delivers the electroporation stimulus train. This technique allows the experimenter to target cells of their choice at a particular time of development and at a particular location in the embryo, and is useful for fate mapping, analysing neuronal organisation, ectopic expression and gene knockdown experiments.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping