PUBLICATION
Imaging zebrafish embryos by two-photon excitation time-lapse microscopy
- Authors
- Carvalho, L., and Heisenberg, C.P.
- ID
- ZDB-PUB-090424-10
- Date
- 2009
- Source
- Methods in molecular biology (Clifton, N.J.) 546: 273-287 (Chapter)
- Registered Authors
- Heisenberg, Carl-Philipp
- Keywords
- Zebrafish, Two-photon excitation microscopy, Time-lapse, Cell migration, YSL nuclei, Gastrulation
- MeSH Terms
-
- Animals
- Cell Movement
- Gastrulation
- Genes, Reporter
- Green Fluorescent Proteins
- Image Processing, Computer-Assisted/methods
- Microscopy, Confocal/instrumentation
- Microscopy, Confocal/methods*
- Morphogenesis
- Photons
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Recombinant Fusion Proteins/biosynthesis
- Recombinant Fusion Proteins/genetics
- Time Factors
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish/metabolism
- PubMed
- 19378110 Full text @ Meth. Mol. Biol.
Citation
Carvalho, L., and Heisenberg, C.P. (2009) Imaging zebrafish embryos by two-photon excitation time-lapse microscopy. Methods in molecular biology (Clifton, N.J.). 546:273-287.
Abstract
The zebrafish is a favorite model organism to study tissue morphogenesis during development at a subcellular level. This largely results from the fact that zebrafish embryos are transparent and thus accessible to various imaging techniques, such as confocal and two-photon excitation (2PE) microscopy. In particular, 2PE microscopy has been shown to be useful for imaging deep cell layers within the embryo and following tissue morphogenesis over long periods. This chapter describes how to use 2PE microscopy to study morphogenetic movements during early zebrafish embryonic development, providing a general blueprint for its use in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping