PUBLICATION
Molecular cloning, ontogeny and tissue distribution of zebrafish (Danio rerio) prohormone convertases: pcsk1 and pcsk2
- Authors
- Morash, M.G., Macdonald, A., Croll, R.P., and Anini, Y.
- ID
- ZDB-PUB-090406-5
- Date
- 2009
- Source
- General and comparative endocrinology 162(2): 179-187 (Journal)
- Registered Authors
- Croll, Roger P.
- Keywords
- Zebrafish, prohormone convertases, pcsk1, pcsk2, Cloning, Ontogeny
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Cloning, Molecular
- Embryo, Nonmammalian/metabolism
- Molecular Sequence Data
- Phylogeny
- Proprotein Convertase 1/chemistry
- Proprotein Convertase 1/genetics*
- Proprotein Convertase 1/metabolism
- Proprotein Convertase 2/chemistry
- Proprotein Convertase 2/genetics*
- Proprotein Convertase 2/metabolism
- RNA, Messenger/chemistry
- RNA, Messenger/metabolism
- Sequence Alignment
- Zebrafish/genetics
- Zebrafish/growth & development
- Zebrafish/metabolism*
- Zebrafish Proteins/chemistry
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- PubMed
- 19332069 Full text @ Gen. Comp. Endocrinol.
Citation
Morash, M.G., Macdonald, A., Croll, R.P., and Anini, Y. (2009) Molecular cloning, ontogeny and tissue distribution of zebrafish (Danio rerio) prohormone convertases: pcsk1 and pcsk2. General and comparative endocrinology. 162(2):179-187.
Abstract
Prohormone convertase subtilisin/kexin (PCSK) enzymes are a family of nine related serine proteases, found in a multitude of tissues, and responsible for the maturation of a variety of protein and peptides precursors. Pcsk1 and Pcsk2 are found within dense core secretory granules in endocrine and neuroendocrine cells and are responsible for cleaving several hormones and neuropetide precursors. In this work, we cloned and sequenced the cDNA of pcsk1 and pcsk2 from zebrafish (Danio rerio). pcsk1 is a 2268 base pair ORF, whose 755 amino acid protein product is identical to that predicted from the genome sequence. pcsk2 is a 1941 base pair ORF, encoding a 646 amino acid peptide. Both Pcsk1 and Pcsk2 display high degrees of similarity to their counterparts in other species, including the conservation of the catalytic triad and other essential residues. The brain contained the highest expression levels of both pcsk1 (1.49 +/- 0.21) (displayed as ratio to EF-1a), and pcsk2 (0.23 +/- 0.04). Both transcripts were also detectable in the fore, mid and distal gut. pcsk1 and 2 were detectable at 4.5 hours post fertilization, and while pcsk1 expression increased throughout development (0.12 +/- 0.01 maximum at 3 days post-fertilization), pcsk2 expression was highest at day 5 post-fertilization (0.03 +/- 0.01), and then decreased thereafter. For the first time, we have identified and characterized a pcsk1 transcript in fish. We have also identified and characterized the pcsk2 transcript in zebrafish, and have assessed the tissue distribution and ontogeny of both.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping