Genomic regions with distinct genomic distance conservation in vertebrate genomes

Sun, H., Skogerbo, G., Zheng, X., Liu, W., and Li, Y.
BMC Genomics   10: 133 (Journal)
Registered Authors
Liu, Wei
MeSH Terms
  • Animals
  • Comparative Genomic Hybridization
  • Conserved Sequence*
  • CpG Islands
  • DNA/genetics
  • Databases, Nucleic Acid
  • Evolution, Molecular*
  • Genome*
  • Genome, Human
  • Genomics
  • Humans
  • INDEL Mutation
  • Repetitive Sequences, Nucleic Acid
  • Sequence Analysis, DNA
  • Vertebrates/genetics*
19323843 Full text @ BMC Genomics
BACKGROUND: A number of vertebrate highly conserved elements (HCEs) have been detected and their genomic interval distances have been reported to be more conserved than protein coding genes among mammalian genomes. A characteristic of the human - non-mammalian comparisons is a bimodal distribution of relative distance difference of conserved consecutive HCE pairs; and it is difficult to attribute such profile to a random assortment. We therefore undertook an analysis of the human genomic regions confined by consecutive HCE pairs common to eight genomes (human, mouse, rat, chicken, frog, zebrafish, tetradon and fugu). RESULTS: Among HCE pairs, we found that some consistently preserve highly conserved interval distance among genomes while others have relatively low distance conservation. Using a partition method, we detected two groups of inter-HCE regions (IHRs) with distinct distance conservation pattern in vertebrate genomes: IHR1s that are bordered by HCE pairs with relative small distance variation, and IHR2s with larger distance difference values. Compared to random background, annotated repeat sequences are significantly less frequent in IHR1s than IHR2s, which reflects a correlation between repeat sequences and the length expansion of IHRs. Both groups of IHRs are unexpectedly enriched in human indel (i.e. insertion and deletion) polymorphism-variations than random background. The correlation between the percentage of conserved sequence and human IHR length was stronger for IHR1 than IHR2. Both groups of IHRs are significantly enriched for CpG islands. CONCLUSIONS: The data suggest that subsets of HCE pairs may undergo different evolutionary paths in light of their genomic distance conservation, and that sets of genomic regions pertain to HCEs, as well as the region in which HCEs reside, should be treated as integrated domains.
Genes / Markers
Show all Figures
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Engineered Foreign Genes
Errata and Notes