PUBLICATION

Lin9 is required for mitosis and cell survival during early zebrafish development

Authors
Kleinschmidt, M.A., Wagner, T.U., Liedtke, D., Spahr, S., Samans, B., and Gaubatz, S.
ID
ZDB-PUB-090318-10
Date
2009
Source
The Journal of biological chemistry   284(19): 13119-13127 (Journal)
Registered Authors
Liedtke, Daniel, Wagner, Toni
Keywords
none
MeSH Terms
  • Mitosis/physiology*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Embryonic Development/physiology*
  • Zebrafish
  • Gene Expression Regulation, Developmental*
  • Embryo, Nonmammalian/physiology*
  • Cell Cycle Proteins/genetics*
  • Cell Cycle Proteins/metabolism*
  • Animals
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
  • Zebrafish Proteins/physiology*
  • Morpholines/pharmacology
  • RNA, Messenger/genetics
  • RNA, Messenger/metabolism
  • In Situ Hybridization
  • Gene Expression Profiling
  • Cell Survival/physiology
  • Apoptosis
  • Flow Cytometry
  • Oligonucleotide Array Sequence Analysis
(all 21)
PubMed
19278998 Full text @ J. Biol. Chem.
Abstract
LIN9 has been described as a regulator of G1/S and G2/M progression of the cell cycle in invertebrates and human cell lines. To elucidate the in vivo function of LIN9 during vertebrate development, we took advantage of the teleost zebrafish (Danio rerio). By means of antisense morpholinos we show here that Lin9-depleted embryonic cells accumulate in mitosis. Flow cytometry and confocal microscopy data demonstrate that the delay in mitotic progression is followed by apoptosis, which strongly manifests in the developing CNS. In accordance with these findings we identified a cohort of Lin9 regulated genes required for different mitotic processes, including mitotic entry, metaphase/anaphase transition and cytokinesis. Our data establish LIN9 as an essential regulator of mitosis in vertebrate development.
Genes / Markers
Figures
No images available
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
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Citation
Kleinschmidt, M.A., Wagner, T.U., Liedtke, D., Spahr, S., Samans, B., and Gaubatz, S. (2009) Lin9 is required for mitosis and cell survival during early zebrafish development. The Journal of biological chemistry. 284(19):13119-13127.