PUBLICATION

Normal forebrain development may require continual Wnt antagonism until mid-somitogenesis in zebrafish

Authors
Kim, J.D., Chun, H.S., Kim, S.H., Kim, H.S., Kim, Y.S., Kim, M.J., Shin, J., Rhee, M., Yeo, S.Y., and Huh, T.L.
ID
ZDB-PUB-090310-5
Date
2009
Source
Biochemical and Biophysical Research Communications   381(4): 717-721 (Journal)
Registered Authors
Huh, Tae-Lin, Kim, Hyung-Seok, Kim, Sun-You, Shin, Jimann, Yeo, Sang-Yeob
Keywords
Forebrain development, Wnt antagonist, Zygotic one-eyed pinhead (oep) mutant, frizzled8a, Transgenic zebrafish
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Frizzled Receptors/biosynthesis*
  • Homeodomain Proteins/metabolism
  • Prosencephalon/growth & development*
  • Prosencephalon/metabolism
  • Receptors, G-Protein-Coupled/biosynthesis*
  • Somites/growth & development*
  • Somites/metabolism
  • Transcription Factors/metabolism
  • Wnt Proteins/antagonists & inhibitors*
  • Wnt Proteins/metabolism
  • Zebrafish/genetics
  • Zebrafish/growth & development*
  • Zebrafish/metabolism
  • Zebrafish Proteins/biosynthesis*
PubMed
19258008 Full text @ Biochem. Biophys. Res. Commun.
Abstract
During normal forebrain development in vertebrates, rostral neural tissue must be protected from Wnt signals via the actions of locally expressed Wnt antagonistic factors. In zebrafish zygotic oep (Zoep) mutants, forebrain structure is severely disrupted with reduced expression of the Wnt antagonists secreted frizzled related protein1 and dickkopf1. To analyze the temporal effects of Wnt antagonism on forebrain development, we generated transgenic zebrafish that overexpressed the dominant negative form of frizzled8a (DNfz8a) in wild-type and Zoep mutants under the control of a heat-inducible promoter. This model allowed for assessment of the dynamics of Wnt antagonistic signaling during forebrain development. Our results demonstrated that overexpression of DNfz8a in Zoep embryos between 7 and 16hpf increased putative forebrain region demarcated by anf and distal-less2 expressions. These results suggest that normal forebrain development requires continual Wnt antagonism from the early gastrula to the mid-somitogenesis stage.
Genes / Markers
Figures
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Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes