PUBLICATION
Expression of a novel cytochrome P450 4T gene in rare minnow (Gobiocypris rarus) following perfluorooctanoic acid exposure
- Authors
- Liu, Y., Wang, J., Liu, Y., Zhang, H., Xu, M., and Dai, J.
- ID
- ZDB-PUB-090310-28
- Date
- 2009
- Source
- Comparative biochemistry and physiology. Toxicology & pharmacology : CBP 150(1): 57-64 (Journal)
- Registered Authors
- Keywords
- rare minnow, CYP4T11, perfluorooctanoic acid (PFOA), PPARα, PPARγ
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- Caprylates/toxicity*
- Cyprinidae/physiology*
- Cytochrome P-450 Enzyme System/biosynthesis*
- Cytochrome P-450 Enzyme System/genetics*
- DNA, Complementary/biosynthesis
- DNA, Complementary/genetics
- Environmental Exposure/adverse effects
- Female
- Fluorocarbons/toxicity*
- Gene Expression Regulation, Enzymologic/drug effects*
- Gene Expression Regulation, Enzymologic/physiology
- Male
- Molecular Sequence Data
- Phylogeny
- PubMed
- 19258050 Full text @ Comp. Biochem. Physiol. C Toxicol. Pharmacol.
Citation
Liu, Y., Wang, J., Liu, Y., Zhang, H., Xu, M., and Dai, J. (2009) Expression of a novel cytochrome P450 4T gene in rare minnow (Gobiocypris rarus) following perfluorooctanoic acid exposure. Comparative biochemistry and physiology. Toxicology & pharmacology : CBP. 150(1):57-64.
Abstract
Cytochrome P450s play an important role in the biotransformation of endogenous substrates and xenobiotics; however, little is known about the function of the CYP4T subfamily in the transformation of environmental pollutants in fish. We isolated a full-length cDNA sequence (designated as CYP4T11) from rare minnow (Gobiocypris rarus) liver by rapid amplification of cDNA ends. The open reading frame encoded a 467-residue protein that exhibited 87% and 71% identity with zebrafish CYP4T8 and European sea bass CYP4T2, respectively. CYP4T11 was predominantly expressed in liver and intestine with lower expression in the gill and brain. To further examine the function of CYP4T11 in pollutant metabolism, the effects of perfluorooctanoic acid (PFOA) exposure on the transcriptional expression of CYP4T11 and two possible upstream regulators, PPARalpha and PPARgamma, were determined in rare minnow gills and livers. PFOA induced a consistent significant upregulation of both PPARalpha and PPARgamma and a nonsignificant increase of CYP4T11 in the gill. In the liver, induced expression of PPARgamma was observed, although no obvious changes in PPARalpha expression were observed. Induction of CYP4T11 was only observed in males at the highest concentration of PFOA. These results suggest that the PPAR-CYP4T11 signaling pathway may be involved in PFOA-induced gill toxicity. Since the induced expression of CYP4T11 in liver was not consistent with the PPAR regulators, complex tissue-specific transcriptional regulation of CYP4T11 following PFOA exposure likely occurs.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping