PUBLICATION
Development of Skeletal Muscle Sodium and Potassium Currents in Zebrafish sofa potato Mutants
- Authors
- Coutts, C.A., Cunningham, M.E., and Ali, D.W.
- ID
- ZDB-PUB-090310-23
- Date
- 2009
- Source
- Developmental neuroscience 31(3): 212-222 (Journal)
- Registered Authors
- Keywords
- Ion channels, Synaptic activity, Nicotinic acetylcholine receptors, Neuromuscular junction
- MeSH Terms
-
- Animals
- Embryo, Nonmammalian
- Membrane Potentials/physiology
- Muscle, Skeletal/physiology*
- Mutation
- Patch-Clamp Techniques
- Potassium Channels/physiology*
- Receptors, Nicotinic/deficiency*
- Receptors, Nicotinic/genetics
- Sodium Channels/physiology*
- Zebrafish/physiology*
- Zebrafish Proteins/genetics
- PubMed
- 19270440 Full text @ Dev. Neurosci.
Citation
Coutts, C.A., Cunningham, M.E., and Ali, D.W. (2009) Development of Skeletal Muscle Sodium and Potassium Currents in Zebrafish sofa potato Mutants. Developmental neuroscience. 31(3):212-222.
Abstract
In some cells, the development of voltage-gated channels requires synaptic input, while in others it does not. Here we investigate whether the sodium and potassium currents in the skeletal muscle of zebrafish sofa potato (sop(-/-)) mutants develop normally. Zebrafish sop(-/-) mutants do not express nicotinic acetylcholine receptors at neuromuscular junctions, and therefore do not exhibit synaptic activity in muscle. We find that in both red and white muscle fibers, sop(-/-) mutants are able to support normal potassium current development during early stages of development [1-3 days post fertilization (dpf)], but at 6 dpf the potassium current density is significantly smaller than that in their phenotypically wild-type siblings (sop(+/?)). In contrast, sodium current density is unaffected. The steady-state properties of potassium currents are unaltered in the sop(-/-) mutants, but there is a significant difference in the V(50) of inactivation of sodium currents. This is the first study in zebrafish to investigate activity-dependent mechanisms of ion channel development and our results indicate that some aspects of ion current development in skeletal muscle require synaptic activity, whereas others do not.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping