PUBLICATION

Auditory input to CNS is acquired coincidentally with development of inner ear after formation of functional afferent pathway in zebrafish

Authors
Tanimoto, M., Ota, Y., Horikawa, K., and Oda, Y.
ID
ZDB-PUB-090310-10
Date
2009
Source
The Journal of neuroscience : the official journal of the Society for Neuroscience   29(9): 2762-2767 (Journal)
Registered Authors
Oda, Yoichi
Keywords
none
MeSH Terms
  • Acoustic Stimulation
  • Afferent Pathways/growth & development*
  • Animals
  • Animals, Genetically Modified
  • Auditory Pathways/growth & development*
  • Central Nervous System/growth & development*
  • Cochlear Microphonic Potentials/physiology
  • Ear, Inner/growth & development*
  • Electrophysiology
  • Evoked Potentials, Auditory/physiology
  • Immunohistochemistry
  • Neurons/physiology
  • Vestibulocochlear Nerve/growth & development
  • Zebrafish/physiology*
PubMed
19261871 Full text @ J. Neurosci.
Abstract
Auditory perception in vertebrates depends on transduction of sound into neural signals in the inner ear hair cells (HCs) and on transmission of these signals to the brain through auditory (VIIIth) nerve afferents. To investigate the developmental acquisition of auditory inputs by the CNS, we have electrophysiologically and morphologically examined the process of acquisition of auditory responsiveness by zebrafish macular HCs and the Mauthner cells (M-cells) in vivo. The M-cells are a paired large reticulospinal neurons in the hindbrain; they receive direct inputs from the VIIIth nerve afferents and initiate an acoustic startle response. Whole-cell recordings from the M-cells showed that sound-evoked postsynaptic currents were first observed around 40 h postfertilization (hpf); during subsequent development, onset latency decreased and amplitude increased. The appearance and development of microphonic potentials in the inner ear coincided with those of the acoustic responses of the M-cell, whereas the functional auditory circuits from the macular HCs to the M-cell were already formed at 27 hpf. These results suggest that the functional maturation of inner ear after formation of the auditory pathway is a critical process in the acquisition of auditory inputs by CNS neurons.
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