PUBLICATION
Exploring Differential Gene Expression in Zebrafish to Teach Basic Molecular Biology Skills
- Authors
- Schmoldt, A., Forecki, J., Hammond, D.R., and Udvadia, A.J.
- ID
- ZDB-PUB-090309-3
- Date
- 2009
- Source
- Zebrafish 6(2): 187-199 (Other)
- Registered Authors
- Schmoldt, Angela, Udvadia, Ava J.
- Keywords
- none
- MeSH Terms
-
- Animals
- Clinical Laboratory Techniques
- Gene Expression Regulation*
- Molecular Biology/education*
- Universities
- Zebrafish/embryology
- Zebrafish/genetics*
- PubMed
- 19250030 Full text @ Zebrafish
Citation
Schmoldt, A., Forecki, J., Hammond, D.R., and Udvadia, A.J. (2009) Exploring Differential Gene Expression in Zebrafish to Teach Basic Molecular Biology Skills. Zebrafish. 6(2):187-199.
Abstract
In an effort to engage students in original research while teaching them basic molecular biology skills, we have designed a course for upper level undergraduate students and beginning graduate students that employs in situ hybridization in whole-mount zebrafish embryos to explore the concept of differential gene regulation. The course was taught in a workshop format during a break between the normal fall and spring semesters, which allowed students to immerse themselves in the concepts and techniques full time over a 13-day period. Overall, the course was successful in exposing students to a variety of techniques in the context of an ongoing research project in our laboratory, which provided beneficial outcomes for students and instructors alike. Here we provide a detailed account of the course organization and preparation, as well as an analysis of learning outcomes achieved by the students.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping