PUBLICATION
Biophysical properties of zebrafish ether-à-go-go related gene potassium channels
- Authors
- Scholz, E.P., Niemer, N., Hassel, D., Zitron, E., Bürgers, H.F., Bloehs, R., Seyler, C., Scherer, D., Thomas, D., Kathöfer, S., Katus, H.A., Rottbauer, W.A., and Karle, C.A.
- ID
- ZDB-PUB-090302-6
- Date
- 2009
- Source
- Biochemical and Biophysical Research Communications 381(2): 159-164 (Journal)
- Registered Authors
- Hassel, David, Rottbauer, Wolfgang
- Keywords
- zERG, hERG, Potassium channel, Delayed rectifier, Repolarization, Arrhythmia, Animal model
- MeSH Terms
-
- Oocytes
- Zebrafish/genetics
- Zebrafish/physiology*
- Humans
- Membrane Potentials
- Xenopus
- Zebrafish Proteins/agonists
- Zebrafish Proteins/genetics
- Zebrafish Proteins/physiology*
- Animals
- Ether-A-Go-Go Potassium Channels/agonists
- Ether-A-Go-Go Potassium Channels/genetics
- Ether-A-Go-Go Potassium Channels/physiology*
- PubMed
- 19232322 Full text @ Biochem. Biophys. Res. Commun.
Citation
Scholz, E.P., Niemer, N., Hassel, D., Zitron, E., Bürgers, H.F., Bloehs, R., Seyler, C., Scherer, D., Thomas, D., Kathöfer, S., Katus, H.A., Rottbauer, W.A., and Karle, C.A. (2009) Biophysical properties of zebrafish ether-à-go-go related gene potassium channels. Biochemical and Biophysical Research Communications. 381(2):159-164.
Abstract
The zebrafish is increasingly recognized as an animal model for the analysis of hERG-related diseases. However, functional properties of the zebrafish orthologue of hERG have not been analyzed yet. We heterologously expressed cloned ERG channels in Xenopus oocytes and analyzed biophysical properties using the voltage clamp technique. zERG channels conduct rapidly activating and inactivating potassium currents. However, compared to hERG, the half-maximal activation voltage of zERG current is shifted towards more positive potentials and the half maximal steady-state inactivation voltage is shifted towards more negative potentials. zERG channel activation is delayed and channel deactivation is accelerated significantly. However, time course of zERG conducted current under action potential clamp is highly similar to the human orthologue. In summary, we show that ERG channels in zebrafish exhibit biophysical properties similar to the human orthologue. Considering the conserved channel function, the zebrafish represents a valuable model to investigate human ERG channel related diseases.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping