Increased DJ-1 Expression under Oxidative Stress and in Alzheimer's Disease Brains
- Baulac, S., Lu, H., Strahle, J., Yang, T., Goldberg, M.S., Shen, J., Schlossmacher, M.G., Lemere, C.A., Lu, Q., and Xia, W.
- Molecular neurodegeneration 4: 12 (Journal)
- Registered Authors
- Xia, Weiming
- MeSH Terms
- 19243613 Full text @ Mol. Neurodegener.
Baulac, S., Lu, H., Strahle, J., Yang, T., Goldberg, M.S., Shen, J., Schlossmacher, M.G., Lemere, C.A., Lu, Q., and Xia, W. (2009) Increased DJ-1 Expression under Oxidative Stress and in Alzheimer's Disease Brains. Molecular neurodegeneration. 4:12.
Mutations in the DJ-1 gene have been linked to autosomal recessive familial Parkinson's disease. To understand the function of DJ-1, we determined the DJ-1 expression in both zebrafish and post mortem human brains. We found that DJ-1 was expressed early during zebrafish development and throughout adulthood. Knock down (KD) of DJ-1 by injection of morpholino did not cause dramatic morphologic alterations during development, and no loss of dopaminergic neurons was observed in embryos lacking DJ-1. However, DJ-1 KD embryos were more susceptible to programmed cell death. While a slight reduction in staining for islet-1 positive neurons was observed in both DJ-1 KD and H2O2 treated embryos, the number of apoptotic cells was significantly increased in both KD and H2O2 treated embryos. Interestingly, DJ-1 expression was increased in brains of zebrafish under conditions of oxidative stress, indicating that DJ-1 is a part of stress-responsive machinery. Since oxidative stress is one of the major contributors to the development of Alzheimer's disease (AD), we also examined DJ-1 expression in AD brains. Using DJ-1 specific antibodies, we failed to detect a robust staining of DJ-1 in brain tissues from control subjects. However, DJ-1 immunoreactivity was detected in hippocampal pyramidal neurons and astrocytes of AD brains. Therefore, our results strongly suggest that DJ-1 expression is not necessary during zebrafish development but can be induced in zebrafish exposed to oxidative stress and is present in human AD brains.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes