ZFIN ID: ZDB-PUB-090302-2
Usage of putative zebrafish U6 promoters to express shRNA in Nile tilapia and shrimp cell extracts
Boonanuntanasarn, S., Panyim, S., and Yoshizaki, G.
Date: 2009
Source: Transgenic Research   18(3): 323-325 (Journal)
Registered Authors: Boonanuntanasarn, Surintorn
Keywords: Gene knockdown, Zebrafish U6 promoter, Nile tilapia, Shrimp, Expression
MeSH Terms:
  • Animals
  • Cell Extracts
  • Decapoda/genetics
  • Decapoda/metabolism*
  • Gene Expression Regulation
  • Promoter Regions, Genetic*
  • RNA, Small Nuclear/genetics*
  • Tilapia/genetics
  • Tilapia/metabolism*
  • Zebrafish/genetics*
PubMed: 19225901 Full text @ Transgenic. Res.
We conducted in vitro transcription activities of the three zebrafish U6 putative promoters across species in cell extracts prepared from Nile tilapia (Oreochromis niloticus) and shrimps. The transcription efficiency of these putative U6 promoters in Nile tilapia cell extracts was similar to that of zebrafish cell extracts. In addition, all three zebrafish U6 snRNA promoters were able to express the shRNA in cell extracts prepared from two shrimp species, Penaeus monodon and Litopenaeus vannamei. However, the shRNA transcription products in shrimp cell extracts showed weaker signals. These U6 promoters could promote shRNA expression, suggesting that they have the potential for use for vector-based RNAi in Nile tilapia and shrimps. A putative U6 promoter would provide a powerful tool for long-term GKD in these aquaculture-related species.