PUBLICATION
Expression of voltage-activated calcium channels in the early zebrafish embryo
- Authors
- Sanhueza, D., Montoya, A., Sierralta, J., and Kukuljan, M.
- ID
- ZDB-PUB-090227-7
- Date
- 2009
- Source
- Zygote (Cambridge, England) 17(2): 131-135 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Calcium/metabolism
- Calcium Channel Blockers/pharmacology
- Calcium Channels/biosynthesis*
- Calcium Channels/drug effects
- Calcium Channels/genetics
- Dihydropyridines/pharmacology
- Embryo, Nonmammalian/drug effects
- Embryo, Nonmammalian/metabolism
- Embryonic Development/drug effects
- Embryonic Development/physiology*
- Gene Expression Regulation, Developmental
- Ion Channel Gating/drug effects
- Ion Channel Gating/physiology
- Zebrafish/embryology*
- Zebrafish Proteins/biosynthesis*
- Zebrafish Proteins/genetics
- PubMed
- 19222870 Full text @ Zygote
Citation
Sanhueza, D., Montoya, A., Sierralta, J., and Kukuljan, M. (2009) Expression of voltage-activated calcium channels in the early zebrafish embryo. Zygote (Cambridge, England). 17(2):131-135.
Abstract
Increases in cytosolic calcium concentrations regulate many cellular processes, including aspects of early development. Calcium release from intracellular stores and calcium entry through non-voltage-gated channels account for signalling in non-excitable cells, whereas voltage-gated calcium channels (CaV) are important in excitable cells. We report the expression of multiple transcripts of CaV, identified by its homology to other species, in the early embryo of the zebrafish, Danio rerio, at stages prior to the differentiation of excitable cells. CaV mRNAs and proteins were detected as early as the 2-cell stages, which indicate that they arise from both maternal and zygotic transcription. Exposure of embryos to pharmacological blockers of CaV does not perturb early development significantly, although late effects are appreciable. These results suggest that CaV may have a role in calcium homeostasis and control of cellular process during early embryonic development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping