PUBLICATION
A gene regulatory network directed by zebrafish No tail accounts for its roles in mesoderm formation
- Authors
- Morley, R.H., Lachani, K., Keefe, D., Gilchrist, M.J., Flicek, P., Smith, J.C., and Wardle, F.C.
- ID
- ZDB-PUB-090227-12
- Date
- 2009
- Source
- Proceedings of the National Academy of Sciences of the United States of America 106(10): 3829-3834 (Journal)
- Registered Authors
- Smith, Jim, Wardle, Fiona
- Keywords
- brachyury, chromatin immunopreciptitation, microarray
- Datasets
- GEO:GSE12331
- MeSH Terms
-
- Animals
- Base Sequence
- Binding Sites
- Body Patterning/genetics
- Cell Lineage
- Conserved Sequence
- Fetal Proteins/genetics
- Fetal Proteins/metabolism*
- Gastrulation/genetics
- Gene Expression Regulation, Developmental
- Gene Regulatory Networks*
- Homeodomain Proteins/genetics
- Homeodomain Proteins/metabolism
- Mesoderm/embryology*
- Molecular Sequence Data
- Muscles/cytology
- Protein Binding
- T-Box Domain Proteins/genetics
- T-Box Domain Proteins/metabolism*
- Transcription Factors/genetics
- Transcription Factors/metabolism
- Transcription, Genetic
- Zebrafish/embryology*
- Zebrafish/genetics*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 19225104 Full text @ Proc. Natl. Acad. Sci. USA
Citation
Morley, R.H., Lachani, K., Keefe, D., Gilchrist, M.J., Flicek, P., Smith, J.C., and Wardle, F.C. (2009) A gene regulatory network directed by zebrafish No tail accounts for its roles in mesoderm formation. Proceedings of the National Academy of Sciences of the United States of America. 106(10):3829-3834.
Abstract
Using chromatin immunoprecipitation combined with genomic microarrays we have identified targets of No tail (Ntl), a zebrafish Brachyury ortholog that plays a central role in mesoderm formation. We show that Ntl regulates a downstream network of other transcription factors and identify an in vivo Ntl binding site that resembles the consensus T-box binding site (TBS) previously identified by in vitro studies. We show that the notochord-expressed gene floating head (flh) is a direct transcriptional target of Ntl and that a combination of TBSs in the flh upstream region are required for Ntl-directed expression. Using our genome-scale data we have assembled a preliminary gene regulatory network that begins to describe mesoderm formation and patterning in the early zebrafish embryo.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping