PUBLICATION

Multiple rapid progestin actions and progestin membrane receptor subtypes in fish

Authors
Thomas, P., Pang, Y., Zhu, Y., Detweiler, C., and Doughty, K.
ID
ZDB-PUB-090225-5
Date
2004
Source
Steroids   69(8-9): 567-573 (Journal)
Registered Authors
Zhu, Yong
Keywords
Progestin membrane receptors, Rapid progestin actions, Oocyte maturation, Sperm motility, Neuroendocrine function, Fish
MeSH Terms
  • Animals
  • Anterior Hypothalamic Nucleus/drug effects
  • Anterior Hypothalamic Nucleus/metabolism
  • Down-Regulation
  • Female
  • Gonadotropin-Releasing Hormone
  • Male
  • Oocytes/metabolism
  • Perciformes/physiology*
  • Preoptic Area/drug effects
  • Preoptic Area/metabolism
  • Progestins/physiology*
  • Protein Isoforms/drug effects
  • Protein Isoforms/genetics
  • Protein Isoforms/metabolism
  • Receptors, Progesterone/drug effects
  • Receptors, Progesterone/genetics
  • Receptors, Progesterone/metabolism
  • Receptors, Progesterone/physiology*
  • Recombinant Proteins/drug effects
  • Recombinant Proteins/genetics
  • Recombinant Proteins/metabolism
  • Sperm Motility/drug effects
  • Zebrafish/physiology*
PubMed
15288771 Full text @ Steroids
Abstract
Progestin hormones exert rapid, nongenomic actions on a variety of target tissues in fish. The induction of oocyte maturation and the progestin membrane receptor (mPR) that mediates this action of progestins have been well characterized in fishes. Progestins also act on Atlantic croaker spermatozoa via an mPR to rapidly increase sperm motility. Preliminary results indicate that progestins can also exert rapid actions in the preoptic anterior hypothalamus (POAH) in this species to down-regulate gonadotropin-releasing hormone (GnRH) secretion. Recently, we reported the cloning, sequencing and characterization of a novel cDNA in a closely related species, spotted seatrout, that has the characteristics of the mPR involved in the progestin induction of oocyte maturation. Three distinct mPR subtypes, named alpha, beta, and gamma, have been identified in both fishes and mammals. The tissue distribution of the mPRalpha protein in seatrout suggests the alpha-subtype mediates progestin actions on GnRH secretion, sperm motility and oocyte maturation. However, mPRbeta antisense experiments in zebrafish oocytes suggest the beta-subtype also participates in the control of oocyte maturation in zebrafish.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping