PUBLICATION
Molecular cloning and expression analysis of a macrophage-colony stimulating factor receptor-like gene from rainbow trout, Oncorhynchus mykiss
- Authors
- Honda, T., Nishizawa, T., Uenobe, M., Kohchi, C., Kuroda, A., Ototake, M., Nakanishi, T., Yokomizo, Y., Takahashi, Y., Inagawa, H., and Soma, G.
- ID
- ZDB-PUB-090225-3
- Date
- 2005
- Source
- Molecular immunology 42(1): 1-8 (Journal)
- Registered Authors
- Keywords
- M-CSF, M-CSFR, Ovary, Reproduction, Teleost fish
- MeSH Terms
-
- Animals
- Base Sequence
- Cloning, Molecular*
- Female
- Gene Expression Profiling*
- Gene Library
- Molecular Sequence Data
- Oncorhynchus mykiss/genetics*
- Open Reading Frames
- Ovary/metabolism
- Receptor, Macrophage Colony-Stimulating Factor/genetics*
- Sequence Alignment
- Sequence Homology, Nucleic Acid
- Tissue Distribution/genetics
- PubMed
- 15488938 Full text @ Mol. Immunol.
Citation
Honda, T., Nishizawa, T., Uenobe, M., Kohchi, C., Kuroda, A., Ototake, M., Nakanishi, T., Yokomizo, Y., Takahashi, Y., Inagawa, H., and Soma, G. (2005) Molecular cloning and expression analysis of a macrophage-colony stimulating factor receptor-like gene from rainbow trout, Oncorhynchus mykiss. Molecular immunology. 42(1):1-8.
Abstract
The M-CSF and its receptor (M-CSFR, CSF-1R or c-fms proto-oncogene) system were initially implicated as essential in mammals for normal monocyte development as well as for pregnancy. To allow a comparison with the M-CSF and M-CSFR system of an oviparous animal, we cloned a M-CSFR-like gene from rainbow trout (Oncorhynchus mykiss). The gene was cloned from a cDNA library of head kidney. It contained an open reading frame encoding 967 amino acids with a predicted size of 109 kDa. The putative amino acid sequence of rainbow trout M-CSFR showed 54% amino acid identity to fugu (Takifugu rubripes) M-CSFR, 52% to zebrafish (Danio rerio) M-CSFR and 40% to mouse (Mus musculus) and human (Homo sapiens) M-CSFR. The M-CSFR-like gene was constitutively expressed in head kidney, kidney, intestine, spleen and blood. The gene was detected especially in the ovary of immature female rainbow trout. These results suggest that a M-CSFR-like receptor may be involved in female reproductive tracts even in an oviparous animal like fish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping