PUBLICATION
Constitutive expression of three novel catfish CXC chemokines: homeostatic chemokines in teleost fish
- Authors
- Baoprasertkul, P., He, C., Peatman, E., Zhang, S., Li, P., and Liu, Z.
- ID
- ZDB-PUB-090225-2
- Date
- 2005
- Source
- Molecular immunology 42(11): 1355-1366 (Journal)
- Registered Authors
- Keywords
- CXCL2, CXCL12, CXCL14, Chemokine, Fish, Cytokine, Infection, Gene expression, Catfish, Inflammation, Homeostatic
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- Chemokines, CXC/genetics*
- DNA, Complementary/genetics
- Edwardsiella ictaluri/pathogenicity
- Enterobacteriaceae Infections/genetics
- Enterobacteriaceae Infections/immunology
- Enterobacteriaceae Infections/veterinary
- Female
- Fish Diseases/genetics
- Fish Diseases/immunology
- Gene Dosage
- Gene Expression Regulation, Developmental
- Homeostasis
- Humans
- Ictaluridae/genetics*
- Ictaluridae/growth & development
- Ictaluridae/immunology*
- Molecular Sequence Data
- Phylogeny
- PubMed
- 15950731 Full text @ Mol. Immunol.
Citation
Baoprasertkul, P., He, C., Peatman, E., Zhang, S., Li, P., and Liu, Z. (2005) Constitutive expression of three novel catfish CXC chemokines: homeostatic chemokines in teleost fish. Molecular immunology. 42(11):1355-1366.
Abstract
Chemokines are best known for their vital role in leukocyte chemotaxis, as part of the larger inflammatory response. Expression analysis and functional characterization of chemokines in mammalian species have often overlooked the role of these proteins under homeostatic conditions. Recent investigations of chemokine diversity in teleost fish have also centered on the immune-related functions of chemotactic cytokines, such as CXCL8 and CXCL10. While a disease-based approach to chemokines is essential to the development of remediative therapies for both human and animal infections, it may be a poor measure of the overall complexity of chemokine functions. As part of a larger effort to assess the conservation of chemokine diversity in teleost fish, we report here the identification of three novel, constitutively expressed CXC chemokines from channel catfish (Ictalurus punctatus). Phylogenetic analyses indicated that two of the three CXC chemokines were orthologues for mammalian CXCL12 and CXCL14, respectively. Whereas a clear orthology could not yet be established for the third CXC chemokine, it shared highest amino acid identity with mammalian CXCL2. All three CXC chemokines show expression in a wide range of tissues, and early expression during development was observed for CXCL12. The expression of this new set of catfish CXC chemokines was not induced during challenge by infection of Edwardsiella ictaluri, the causative agent of the fish pathogen enteric septicemia of catfish. In contrast to the gene duplication of CXCL12 in carp and zebrafish, Southern blot analysis indicated that all three catfish CXC chemokines exist as single copy genes in the catfish genome suggesting that gene duplication of CXC chemokines in specific teleost fish was a recent evolutionary event.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping