PUBLICATION
FTZ-F1alpha is expressed in the developing gonad of frogs
- Authors
- Takase, M., Nakajima, T., and Nakamura, M.
- ID
- ZDB-PUB-090210-19
- Date
- 2000
- Source
- BBA Gene Structure and Expression 1494(1-2): 195-200 (Journal)
- Registered Authors
- Keywords
- Frog, Gonad, FTZ-F1 protein, In situ hybridization
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- Cell Differentiation
- DNA-Binding Proteins/chemistry
- DNA-Binding Proteins/genetics*
- Female
- Fushi Tarazu Transcription Factors
- Gene Expression Regulation, Developmental
- Germ Cells/cytology
- Germ Cells/metabolism
- Gonads/cytology
- Gonads/growth & development*
- Gonads/metabolism*
- Homeodomain Proteins
- Humans
- In Situ Hybridization
- Male
- Metamorphosis, Biological/genetics
- Molecular Sequence Data
- Ovary/cytology
- Ovary/growth & development
- Ovary/metabolism
- RNA, Messenger/analysis
- RNA, Messenger/genetics
- Ranidae/genetics*
- Ranidae/growth & development*
- Receptors, Cytoplasmic and Nuclear
- Sequence Alignment
- Steroidogenic Factor 1
- Testis/cytology
- Testis/growth & development
- Testis/metabolism
- Transcription Factors/chemistry
- Transcription Factors/genetics*
- PubMed
- 11072086 Full text @ BBA Gene Structure and Expression
Citation
Takase, M., Nakajima, T., and Nakamura, M. (2000) FTZ-F1alpha is expressed in the developing gonad of frogs. BBA Gene Structure and Expression. 1494(1-2):195-200.
Abstract
Fushi tarazu transcription factor-1 (FTZ-F1), a member of the nuclear hormone receptor superfamily, is a regulator for fushi tarazu gene expression in Drosophila. Its expression pattern during organogenesis in vertebrates, however, is not known yet. In this study, we cloned a frog FTZ-F1 homologue (rrFTZ-F1alpha) and analyzed its expression and localization during gonadal development of the frog Rana rugosa. Cloned rrFTZ-F1alpha cDNA encoded a protein of 501 amino acids including the regions I-III and FTZ-F1 box that are evolutionally conserved in the FTZ-F1 superfamily. rrFTZ-F1alpha shared high similarity at the amino acid level with mouse LRH-1 (76%), human FTF (92%), chicken OR2.0 (92%), Xenopus laevis FF1rA (94%) and zebrafish FF1A (82%). Northern blot analysis showed that the rrFTZ-F1alpha mRNA at a size of 7.4 kb was the most prominent in the testis among various tissues of adult frogs examined. The RT-PCR analysis revealed that the expression of rrFTZ-F1alpha was weak in the gonad of tadpoles before stage XVI, but it became stronger in the testis of froglets at stage XXV and much higher in the testis of frogs 2 months after metamorphosis. In addition, in situ hybridization analysis revealed that the rrFTZ-F1alpha gene was transcribed in germ cells except for sperm in the testis, and in oocytes at stage A in the ovary of frogs 2 months after metamorphosis. Together, these results suggest that FTZ-F1alpha probably plays an important role in differentiation of germ cells in the gonad of frogs in both sexes.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping