Fluorescence-activated cell sorting (FACS) of whole mount in situ hybridization (WISH) labelled haematopoietic cell populations in the zebrafish
- Dobson, J.T., Da'as, S., McBride, E.R., and Berman, J.N.
- British journal of haematology 144(5): 732-735 (Journal)
- Registered Authors
- Berman, Jason
- zebrafish, FACS, haematopoiesis, flow cytometry, morphology
- MeSH Terms
- Flow Cytometry/methods
- Hematopoietic Stem Cells/cytology*
- In Situ Hybridization/methods
- Models, Animal
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- 19133983 Full text @ Br. J. Haematol.
Dobson, J.T., Da'as, S., McBride, E.R., and Berman, J.N. (2009) Fluorescence-activated cell sorting (FACS) of whole mount in situ hybridization (WISH) labelled haematopoietic cell populations in the zebrafish. British journal of haematology. 144(5):732-735.
The zebrafish is a robust animal model for studying vertebrate haematopoiesis and immune cell interactions. However, fluorescence activated cell sorting (FACS) has been limited due to a paucity of available functional zebrafish antibodies. We have developed a technique combining FACS with whole mount in situ hybridization (WISH) that enables the sorting and examining of fixed zebrafish blood cell populations at different stages of embryonic development, providing the opportunity to correlate RNA expression data with cellular morphology.
Genes / Markers
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Engineered Foreign Genes
Errata and Notes